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Preparation of Organic-Silica Hybrid Monolith with Anion Exchange/Hydrophilic Interaction Mixed-Mode Via Epoxy–Amine Ring-Opening Polymerization Using Polyethylenimine as Functional Monomer

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Abstract

Polyethylenimine (PEI) and 2,4,6,8-tetramethyl-2,4,6,8-tetrakis(propyl glycidyl ether)cyclotetrasiloxane (POSS–epoxy) were used as precursors for the preparation of organic-silica hybrid monolithic columns (PEI–POSS monolith) via epoxy–amine ring-opening polymerization (ROP). The high density of amine groups in PEI provides rich chromatographic interaction sites for the polar or acidic analytes in hydrophilic interaction (HILIC) and weak anion exchange (WAX) mechanisms. The column preparation conditions, such as the porogens, solvent and reaction temperature, were systematically investigated according to the morphology, permeability and column efficiency. The separation mechanisms of HILIC and WAX were evaluated with neutral polar compounds and halogen benzoic acids. Owing to the existence of reactive amine groups on the matrix surface, the PEI–POSS monolith is also an ideal starting material for the preparation of HILIC or strong anionic exchange (SAX) stationary phases by modification. The modification of PEI–POSS monoliths with iodomethane or bromoacetic acid via the nucleophilic substitution reaction could achieve the retention mechanisms of SAX or zwitterionic HILIC, respectively.

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Acknowledgments

Financial support from the National Natural Sciences Foundation of China (no. 21205058) and the starting funding from Dalian University of Technology (no. DUT15RC(3)048) are gratefully acknowledged.

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Authors and Affiliations

  1. School of Food and Environment, Dalian University of Technology, Panjin, China

    Liang Wang, Minghuo Wu, Qixue Wang & Jingjing Zhan

  2. School of Petroleum and Chemical Engineering, Dalian University of Technology, Panjin, China

    Liang Wang & Hongbo Chen

Authors
  1. Liang Wang
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  2. Minghuo Wu
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  3. Qixue Wang
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  4. Jingjing Zhan
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  5. Hongbo Chen
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Correspondence to Minghuo Wu.

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This article does not contain any studies with human participants or animal performed by any of the authors.

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Wang, L., Wu, M., Wang, Q. et al. Preparation of Organic-Silica Hybrid Monolith with Anion Exchange/Hydrophilic Interaction Mixed-Mode Via Epoxy–Amine Ring-Opening Polymerization Using Polyethylenimine as Functional Monomer. Chromatographia 79, 1263–1269 (2016). https://doi.org/10.1007/s10337-016-3150-9

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  • DOI: https://doi.org/10.1007/s10337-016-3150-9

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