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Separation of Epigallocatechin Gallate from Natural Plant Extracts Using Crowding Agents—Assisted Imprinted Polymers

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Abstract

A methodology-based molecularly imprinted polymer (MIP) was proposed to separate epigallocatechin gallate (EGCG) from natural plant extracts. A molecular crowding agent was introduced to improve the affinity and recognizing ability of EGCG in the preparation of an MIP monolith. Acrylamide was used as the functional monomer, and ethylene glycol dimethacrylate was the crosslinking monomer, using a solution of polystyrene in tetrahydrofuran as a molecular crowding agent with isooctane as a coporogen. In addition, it was found that the ratio of the macromolecule agent, the amount of templates and crosslinking degree, and the composition of the mobile phase greatly affected the retention of the template and performance of molecular recognition. The imprinting factor of the resulting MIP monolith obtained was up to 9.67. The resulting MIP can be used to purify EGCG from crude tea polyphenol efficiently with mean recoveries of 87.42 %.

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References

  1. Song JM, Lee KH, Seong BL (2005) Antivir Res 68:66–74

    Article  CAS  Google Scholar 

  2. Tang SZ, Kerry JP, Sheehan D, Buckley DJ (2002) Food Chem 76:45–51

    Article  CAS  Google Scholar 

  3. Fujimura Y, Tachibana H, Yamada K (2004) FEBS Lett 556:204–210

    Article  CAS  Google Scholar 

  4. Chen D, Wan SB, Yang H, Yuan J, Chan TH, Dou QP (2011) Adv Clin Chem 53:155–177

    Article  CAS  Google Scholar 

  5. Cao X, Ito Y (2004) J Liq Chromatogr Rel Tech 27:145–152

    Article  CAS  Google Scholar 

  6. Horie H, Mukai T, Kohata K (1997) J Chromatogr A 758:332–335

    Article  CAS  Google Scholar 

  7. Peres RG, Tonin FG, Tavares MFM, Rodriguez-Amaya DB (2011) Food Chem 127:651–655

    Article  CAS  Google Scholar 

  8. Xu J, Zhang G, Tan T, Janson JC (2005) J Chromatogr B 824:323–326

    Article  CAS  Google Scholar 

  9. Xu J, Tan TW, Janson JC (2007) J Chromatogr A 1169:235–238

    Article  CAS  Google Scholar 

  10. Hong SB, Kim JI, Row KH (2002) Sep Sci Technol 37:1631–1640

    Article  CAS  Google Scholar 

  11. Wang SY, Liang Y, Zheng SW (2012) J Chromatogr A 1265:46–51

    Article  CAS  Google Scholar 

  12. Whitcombe MJ, Kirsch N, Nicholls IA (2014) J Mol Recognit 27:297–401

    Article  CAS  Google Scholar 

  13. Tiwari MP, Prasad A (2015) Anal Chim Acta 853:1–18

    Article  CAS  Google Scholar 

  14. Huang YP, Zheng C, Liu ZS (2011) Curr Org Chem 15:1863–1870

    Article  CAS  Google Scholar 

  15. Ge Y, Butler B, Mirza F, Habib-Ullah S, Fei D (2013) Macromol Rapid Commun 34:903–915

    Article  CAS  Google Scholar 

  16. Tóth B, Horvai G (2012) Top Curr Chem 325:267–306

    Article  Google Scholar 

  17. Duan Y, Luo X, Qin Y, Zhang H, Sun G, Sun X, Yan Y (2013) J Appl Polym Sci 129:2882–2890

    Article  CAS  Google Scholar 

  18. Chen S, Luo Z, Ma X, Xue L, Lan H, Zhang W (2012) Anal Lett 45:2300–2309

    Article  CAS  Google Scholar 

  19. Haginaka J, Tabo H, Ichitani M, Takihara T, Sugimoto A, Sambe H (2007) J Chromatogr A 1156:45–50

    Article  CAS  Google Scholar 

  20. Matsui J, Goji S, Murashima T, Miyoshi D, Komai S, Shigeyasu A, Kushida T, Miyazawa T, Yamada T, Tamaki K, Sugimoto N (2007) Anal Chem 79:1749–1757

    Article  CAS  Google Scholar 

  21. Li XX, Bai LH, Wang H, Wang J, Huang YP, Liu ZS (2012) J Chromatogr A 1251:141–147

    Article  CAS  Google Scholar 

  22. Shi XX, Xu L, Duan HQ, Huang YP, Liu ZS (2011) Electrophoresis 32:1348–1356

    Article  CAS  Google Scholar 

  23. Li XX, Hao LF, Huang YP, Duan HQ, Liu ZS (2012) Polym Eng Sci 52:1440–1449

    Article  CAS  Google Scholar 

  24. Mu LN, Wang XH, Zhao L, Huang YP, Liu ZS (2011) J Chromatogr A 1218:9236–9243

    Article  CAS  Google Scholar 

  25. Ban L, Zhao L, Deng BL, Huang YP, Liu ZS (2013) Anal Bioanal Chem 405:2245–2253

    Article  CAS  Google Scholar 

  26. Zheng C, Huang YP, Liu ZS (2013) Anal Bioanal Chem 405:2147–2161

    Article  CAS  Google Scholar 

  27. Wulff G (2002) Chem Rev 102:1–27

    Article  CAS  Google Scholar 

  28. Andersson HS, Karlsson JG, Piletsky SA, Koch-Schmidt AC, Mosbach K, Nicholls IA (1999) J Chromatogr A 848:39–49

    Article  CAS  Google Scholar 

  29. Schweitz L, Andersson LI, Nilsson S (1997) Anal Chem 69:1179–1183

    Article  CAS  Google Scholar 

  30. Mayes AG, Whitcombe MJ (2005) Adv Drug Deliv Rev 57:1742–1778

    Article  CAS  Google Scholar 

  31. Wulff G (1995) Angew Chem Int Ed Engl 34:1812–1832

    Article  CAS  Google Scholar 

  32. Mayes AG, Mosbach K (1996) Anal Chem 68:3769–3774

    Article  CAS  Google Scholar 

  33. Sellergren B (2001) J Chromatogr A 906:227–252

    Article  CAS  Google Scholar 

  34. Nicholls IA, Ramstrӧm O, Mosbach K (1995) J Chromatogr A 691:349–353

    Article  CAS  Google Scholar 

  35. Tian M, Zhang H, Row KH (2012) Asian J Chem 24:4606–4610

    CAS  Google Scholar 

  36. Zhang HH, Qin Y, Duan YQ, Xu FF, Zhang C, Yan YS, Ma HL, Wang YH (2013) Asian J Chem 25:3711–3718

    CAS  Google Scholar 

  37. Lei QF, Zhong SA, Zhou CS, Yu D (2005) Chin J Anal Chem 6:857–860

    Google Scholar 

Download references

Acknowledgments

This work was financially supported by the High Technology Research and Development Program of Xinjiang (no. 201217149).

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    Authors and Affiliations

    1. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China

      Xuan Sun, Chen Zhang, Yan-Ping Huang & Zhao-Sheng Liu

    2. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, China

      Zhao-Sheng Liu

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    1. Xuan Sun
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    2. Chen Zhang
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    3. Yan-Ping Huang
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    4. Zhao-Sheng Liu
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    Correspondence to Yan-Ping Huang or Zhao-Sheng Liu.

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    X. Sun and C. Zhang contributed equally to this work.

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    Sun, X., Zhang, C., Huang, YP. et al. Separation of Epigallocatechin Gallate from Natural Plant Extracts Using Crowding Agents—Assisted Imprinted Polymers. Chromatographia 78, 995–1003 (2015). https://doi.org/10.1007/s10337-015-2914-y

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    • DOI: https://doi.org/10.1007/s10337-015-2914-y

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