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Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase

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Abstract

In this work, a core–shell molecularly imprinted polymer (MIP) was synthesized through sol–gel coating procedure by using silica beads, emodin, 3-aminopropyltriethoxysilane, tetraethoxysilane and tetrahydrofuran as supporting matrix, template, functional monomer, cross-linker and solvent, respectively. The selective recognition property for emodin and its analogues (physcion and aloe emodin) of the resultant MIP was evaluated in a chromatographic column for high-performance liquid chromatography (HPLC). The retention time and imprinted factor of MIP column for emodin, physcion and aloe emodin were 5.11, 0.63, 0.69 min and 4.69, 0.75, 1.38, respectively. It showed that the MIP beads had a good binding ability for emodin. Finally, one-step separation of emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale was achieved and 380 mg of emodin was collected in 5 days. The product was characterized by mass spectrometry and HPLC and its purity was 95 %.

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Acknowledgments

This work was financially supported by National Key Special Project of Science and Technology for Innovation Drugs (Grant Nos. 2008ZX09401-006, 2009ZX09502-015 and 2012ZX09501001-004) and the country support National science and technology project (2008BAI51BOO).

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Authors and Affiliations

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100102, China

    Tengfei Chen & Wei Xiao

  2. Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Material Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    Tengfei Chen, Jiangyong Gu, Hao Wang, Gu Yuan, Lirong Chen & Xiaojie Xu

  3. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Pharmaceutical Co., Ltd, Lianyungang, 222001, China

    Tengfei Chen & Wei Xiao

Authors
  1. Tengfei Chen
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  2. Jiangyong Gu
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  3. Hao Wang
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  4. Gu Yuan
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  5. Lirong Chen
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  6. Xiaojie Xu
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  7. Wei Xiao
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Corresponding author

Correspondence to Wei Xiao.

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T. Chen and J. Gu made equal contributions to this work.

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Chen, T., Gu, J., Wang, H. et al. Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase. Chromatographia 77, 893–899 (2014). https://doi.org/10.1007/s10337-014-2691-z

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  • DOI: https://doi.org/10.1007/s10337-014-2691-z

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