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Low-Pressure Chromatographic Separation of p-Hydroxybenzoates Using Sequential Injection with Lab-on-Valve System and Miniature Monolithic Column

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Abstract

The use of a small guard column (5-mm length) as a miniature analytical column in low-pressure liquid chromatography based on a sequential injection with lab-on-valve system was demonstrated. A strong initial mobile phase was used to rapidly deliver the sample zone to the column prior to simultaneous separation of analytes with weaker mobile phase. Separation of methyl-, ethyl-, propyl-, and butyl-4-hydroxybenzoates (MP, EP, PP, and BP) was achieved within about 120 s with sufficient peak resolution (all higher than 1.3) using 2,500 µL of mobile phase (composed of 1,087 µL of MeOH) per run. Detection limits were found to be 6.5, 8.0, 0.5, and 0.6 μmol L−1 for MP, EP, PP, and BP, respectively. The system was tested with common commercially available skin lotions and wet wipe products. The analysis results were in good agreement with those obtained from the high-performance liquid chromatography with a 10-cm-length packed column.

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Acknowledgments

Support from the College of Arts and Sciences, Xavier University through the summer research fund for SKH and AK, and from the Commission on Higher Education (CHE) and The Thailand Research Fund (TRF), Thailand for SL are acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Xavier University, 3800 Victory Parkway, Cincinnati, OH, 45207, USA

    Supaporn Kradtap Hartwell & Alison Kehling

  2. Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, Chiang Mai, 50200, Thailand

    Somchai Lapanantnoppakhun

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  1. Supaporn Kradtap Hartwell
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  2. Alison Kehling
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  3. Somchai Lapanantnoppakhun
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Correspondence to Supaporn Kradtap Hartwell.

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Kradtap Hartwell, S., Kehling, A. & Lapanantnoppakhun, S. Low-Pressure Chromatographic Separation of p-Hydroxybenzoates Using Sequential Injection with Lab-on-Valve System and Miniature Monolithic Column. Chromatographia 77, 883–891 (2014). https://doi.org/10.1007/s10337-014-2697-6

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  • DOI: https://doi.org/10.1007/s10337-014-2697-6

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