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The Development of the In Situ Modification of 1st Generation Analytical Scale Silica Monoliths

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Abstract

Analytical scale silica monoliths are commercially limited to three column selectivities (bare silica, C8 and C18). An in situ modification is reported in detail to overcome this barrier and allow for any functionality of choice to be bonded to the silica surface of the monolithic stationary phase support. The modification method was conducted on a commercial bare silica column to bond the C18 moiety to the silica surface through a silylation reaction. The C18 type of stationary phase was chosen, as this is the most commonly bonded functionality for the majority of stationary phases used for high-performance liquid chromatography (HPLC) separations. The C18-modified monolith’s performance was compared to a commercial C18 monolithic and a particle packed column of the same analytical scale column dimensions (100 × 4.6 mm). The modified C18 monolith proved to be of high quality with an efficiency of 73,267 N m−1, fast analysis times (operated at flow rates up to 3 mL min−1 using a conventional 400 bar HPLC system) and improved resolution of a set of polar and non-polar substituted aromatics in comparison to a commercial C18 monolith.

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Acknowledgments

This work was supported by the Australian Research Council’s Discovery funding scheme (DP0987318). E.F.H. is the recipient of an ARC Future Fellowship (FT0990521). We gratefully acknowledge Dr. Thomas Rodemann (Central Science Laboratory, University of Tasmania) for assistance with elemental analysis.

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Authors and Affiliations

  1. Australian Centre for Research on Separation Science (ACROSS), University of Western Sydney, Parramatta, NSW, Australia

    Arianne Soliven, Gary R. Dennis & R. Andrew Shalliker

  2. Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Hobart, TAS, Australia

    Emily F. Hilder

  3. Centre for Chemistry and Biotechnology, Faculty of Science, Engineering and Build Environment, Deakin University, Geelong, VIC, Australia

    Paul G. Stevenson

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  1. Arianne Soliven
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  2. Gary R. Dennis
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  3. Emily F. Hilder
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  4. R. Andrew Shalliker
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  5. Paul G. Stevenson
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Correspondence to Arianne Soliven.

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Soliven, A., Dennis, G.R., Hilder, E.F. et al. The Development of the In Situ Modification of 1st Generation Analytical Scale Silica Monoliths. Chromatographia 77, 663–671 (2014). https://doi.org/10.1007/s10337-014-2667-z

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  • DOI: https://doi.org/10.1007/s10337-014-2667-z

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