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Simultaneous Determination of Eight Typical Biogenic Amines by CZE with Capacitively Coupled Contactless Conductivity Detection

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Abstract

A novel method has been developed for simultaneous determination of eight typical biogenic amines (BAs) based on CZE with capacitively coupled contactless conductivity detection (CZE–C4D). On-column C4D was used for direct quantification of these nonUV-absorbing amine compounds without derivatization. The effects of various experimental factors on separation and detection were investigated. Under the optimum conditions, spermine, spermidine, histamine, putrescine, cadaver, β-phenylethylamine, tyramine, and tryptamine can be well separated within 24 min at the separation voltage of 16 kV in 150 mmol L−1 18-crown-6/500 mmol L−1 acetic acid running buffer, and the excitation voltage and frequency of C4D were 60 V and 550 kHz, respectively. A good linear relationship could be obtained between the peak area and the concentration of each BA at three orders of magnitude; the limits of detection were in the range of 44.3–149 ng mL−1. This proposed method has been successfully applied for the analysis of BAs in water and hard liquor samples.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (No. 21205042), the Special Funds for the Development of Major Scientific Instruments and Equipment (No. 2011YQ15007205).

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People’s Republic of China

    Wenli Li, Yali Pan, Yan Liu, Xiaoli Zhang, Jiannong Ye & Qingcui Chu

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  1. Wenli Li
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  2. Yali Pan
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  3. Yan Liu
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  4. Xiaoli Zhang
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  5. Jiannong Ye
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  6. Qingcui Chu
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Correspondence to Qingcui Chu.

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Li, W., Pan, Y., Liu, Y. et al. Simultaneous Determination of Eight Typical Biogenic Amines by CZE with Capacitively Coupled Contactless Conductivity Detection. Chromatographia 77, 287–292 (2014). https://doi.org/10.1007/s10337-013-2595-3

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  • DOI: https://doi.org/10.1007/s10337-013-2595-3

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