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Electric Field-Assisted Solid Phase Extraction: Devices, Development and Application with a Cationic Model Compound

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Abstract

This work describes novel devices for electric field-assisted solid phase extraction (E-SPE) and an application was carried out on the antimicrobial marbofloxacin (MAR). Conventional syringe-type SPE cartridges were easily adapted to receive two electrodes that were inserted and positioned below and above the sorbent. The adapted cartridges were coupled to a flow extraction system, which consisted of an electrophoresis power supply, a peristaltic pump and a homemade SPE manifold. These devices were used to apply electric fields during the extraction of MAR from fortified buffer and milk samples. The recovery of MAR was improved (2.3 times) or reduced (4.2 times) in comparison to conventional SPE when the top electrode was used as cathode (E-SPE(−/+)) or anode (E-SPE(+/−)), respectively. The results demonstrated that usual SPE cartridges can be easily and inexpensively adapted for applying an electric field in SPE. It was also concluded that the electric field applied in a usual SPE cartridge can be employed as a new suitable approach to enhance the extraction efficiency of ionic compounds in a complex sample matrix.

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Acknowledgments

The authors gratefully acknowledge the financial support from São Paulo Research Foundation (FAPESP, 2007/07079-0, 2008/03064-1 and 2011/11595-0).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Institute of Chemistry, University of Campinas, P.O. Box 6154, Campinas, SP, 13084-971, Brazil

    Ricardo Mathias Orlando, Jarbas José Rodrigues Rohwedder & Susanne Rath

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  1. Ricardo Mathias Orlando
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  2. Jarbas José Rodrigues Rohwedder
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  3. Susanne Rath
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Correspondence to Susanne Rath.

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Orlando, R.M., Rohwedder, J.J.R. & Rath, S. Electric Field-Assisted Solid Phase Extraction: Devices, Development and Application with a Cationic Model Compound. Chromatographia 77, 133–143 (2014). https://doi.org/10.1007/s10337-013-2565-9

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  • DOI: https://doi.org/10.1007/s10337-013-2565-9

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