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Determination of human erythropoietin by on-line immunoaffinity capillary electrophoresis: a preliminary report

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Abstract

Several CE methodologies have been described for the analysis of rHuEPO in concentrated solutions, but the inherently limited concentration sensitivity of CE precludes the detection of EPO at the levels found in biological fluids. In this work, we have investigated an on-line immunoaffinity solid-phase extraction capillary electrophoresis (IA-CE) methodology for the selective preconcentration of EPO in diluted solutions. The preliminary results obtained using a custom-made immunoaffinity sorbent prepared from an anti-human EPO polyclonal antibody and glutaraldehyde–glass beads show the potential of this novel approach. The summarized findings are discussed in detail as a starting point for our ongoing investigations.

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Acknowledgements

E. Hernández acknowledges the Spanish Ministry of Education, Culture and Sports for a FPU (Formación del Profesorado Universitario) fellowship. This work was supported by the Spanish Ministry of Science and Technology (CTQ2005-04357/BQU) and (DEP2005-00234-C03-01).

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028, Barcelona, Spain

    Fernando Benavente, Elena Hernández, Victoria Sanz-Nebot & José Barbosa

  2. Bioanalytical Drug Metabolism, Johnson & Johnson Pharmaceutical Research & Development, 1000 Route 202, Box 300, OMP-B356, Raritan, NJ, 08869, USA

    Norberto A. Guzman

Authors
  1. Fernando Benavente
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  2. Elena Hernández
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  3. Norberto A. Guzman
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  4. Victoria Sanz-Nebot
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  5. José Barbosa
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Correspondence to Fernando Benavente.

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Benavente, F., Hernández, E., Guzman, N.A. et al. Determination of human erythropoietin by on-line immunoaffinity capillary electrophoresis: a preliminary report. Anal Bioanal Chem 387, 2633–2639 (2007). https://doi.org/10.1007/s00216-007-1119-0

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  • DOI: https://doi.org/10.1007/s00216-007-1119-0

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