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Integrated LC/MS and GC/MS Metabolomics Data for the Evaluation of Protection Function of Fructus Ligustri Lucidi on Mouse Liver

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Abstract

A comprehensive metabolomic strategy, integrating GC/MS and LC/MS data, had been developed to study the protection function of herbal medicine, Fructus Ligustri Lucidi, on mouse liver. Mouse plasma samples were analyzed by GC/MS and LC/MS in conjunction with multiblock multivariate analysis method, multiblock partial least squares discriminant analysis (MBPLS-DA), to supply more important and distinct information of metabolomic biomarkers. Then, the biological pathway analysis was carried out to help further understanding the mechanism of liver protection of Fructus Ligustri Lucidi.

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Acknowledgments

The study has been supported by the foundation (Grant No. 81001599) from National Natural Science Foundation of China, the Doctoral Foundation (Grant No. 20103237120010) of Youth Scholars of Ministry of Education of China and the Open Project of National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine (Grant No. 2011ZYX1-009).

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Authors and Affiliations

  1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

    Weifeng Yao, Mu He, Yejuan Jiang, Li Zhang & Anwei Ding

  2. Jiangsu Engineering Laboratory for Research and Industrialization of Empirical Formulae, Nanjing, 210023, China

    Weifeng Yao

  3. Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing, 210023, China

    Mu He, Yejuan Jiang, Li Zhang & Anwei Ding

  4. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, China

    Yuzhu Hu

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  1. Weifeng Yao
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  2. Mu He
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  4. Li Zhang
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Correspondence to Li Zhang or Anwei Ding.

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Yao, W., He, M., Jiang, Y. et al. Integrated LC/MS and GC/MS Metabolomics Data for the Evaluation of Protection Function of Fructus Ligustri Lucidi on Mouse Liver. Chromatographia 76, 1171–1179 (2013). https://doi.org/10.1007/s10337-013-2519-2

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