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Ultrasonic-Assisted Nebulization Extraction Coupled with SPE and HPLC for the Determination of Triterpenoids in Root of Euphorbia pekinensis Rupr.

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Abstract

In this work, a novel method based on ultrasonic-assisted nebulization extraction coupled with solid phase extraction (UANE-SPE) and determined by high performance liquid chromatography was developed for the determination of triterpenoids in root of Euphorbia pekinensis Rupr. The experimental conditions for the UANE-SPE, such as type of extraction solvent, sample amount, type and amount of sorbent, extraction time and volume of the elution solvent, were examined and optimized. The method was successfully applied to determine euphol and tirucallol in the dried root of E. pekinensis Rupr. The recoveries of the analytes were in the range of 89.1–102.0 %. The limits of detection were 12 μg g−1 for tirucallol and 10 μg g−1 for euphol. The extraction yields obtained by the proposed method are higher than those obtained by the conventional extraction methods, such as reflux and ultrasonic-assisted extraction. Compared with the traditional methods, the proposed method can reduce the consumption of the labor, shorten the sample preparation time and increase the efficiencies in the extraction of active constituents from plant materials.

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References

  1. The State Pharmacopoeia Commission of P. R. China (2010) The pharmacopoeia of People’s Republic of China 2010. Chemical Industry Publishing House, Beijing, p 209

  2. The State Pharmacopoeia Commission of P. R. China (2005) The pharmacopoeia of People’s Republic of China 2005. Chemical Industry Publishing House, Beijing, p 156

  3. The State Administration of Traditional Chinese medicine, The Chinese materia medica. Shanghai Science and Technology Press, Shanghai, p 573

  4. Kondo K, Sugi M (1977) Cancer therapy in modern China. Shi Zen Sha, Tokyo, p 254

  5. Xia GC, Li DH (1999) Color atlas of anticancer animals, plants and mineral preparations and their application. Tianjin Science, Technology and Translation Publishing Corp, Tianjin, p 289

    Google Scholar 

  6. Xu GJ, Wang Q, Yu BY, Pan MG (1997) Coloured illustrations of antitumour chinese traditional and herbal drugs. Fujian Science and Technology Press, Fujian, p 218

    Google Scholar 

  7. Zuo Y, Zhang L, Wu J, Fritz JW, Medeiros S, Rego C (2004) Anal Chim Acta 526:35–39

    Article  CAS  Google Scholar 

  8. Rostagno MA, Palma M, Barroso CG (2003) J Chromatogr A 1012:119–128

    Article  CAS  Google Scholar 

  9. Urbanova J, Pencikova K, Gregorova J, Hohnova B, Stavikova L, Karasek P, Roth M, Taborska E (2012) Phytochem Anal 23:477–482

    Article  CAS  Google Scholar 

  10. Shi XL, Jin YR, Liu JB, Zhou HY, Wei W, Zhang HQ, Li XW (2011) Food Chem 129:1253–1257

    Article  CAS  Google Scholar 

  11. Vinatoru M (2001) Ultrason Sonochem 83:303–313

    Article  Google Scholar 

  12. Itoh N, Numata M, Aoyagi Y, Yarita T (2008) Anal Chim Acta 612:44–52

    Article  CAS  Google Scholar 

  13. Wang YT, You JY, Yu Y, Qu CL, Zhang HR, Ding L, Zhang HQ, Li XW (2008) Food Chem 110:161–167

    Article  CAS  Google Scholar 

  14. Arturo LP, Macias MPC (2006) Talanta 69:882–887

    Article  Google Scholar 

  15. Chen LG, Ding L, Zhang HR, Li J, Wang YT, Wang XP, Qu CL, Zhang HQ (2006) Anal Chim Acta 580:75–82

    Article  CAS  Google Scholar 

  16. Wang JX, Xiao XH, Li GK (2008) J Chromatogr A 1198:45–53

    Google Scholar 

  17. Avvaru B, Patil MN, Gogate PR, Pandit AB (2006) Ultrasonics 44:146–158

    Article  CAS  Google Scholar 

  18. Yasuda K, Bando Y, Yamaguchi S, Nakamura M, Oda A, Kawase Y (2005) Ultrason Sonochem 12:37–41

    Article  CAS  Google Scholar 

  19. Takaya H, Nii S, Kawaizumi F, Takahashi K (2005) Ultrason Sonochem 12:483–487

    Article  CAS  Google Scholar 

  20. Jalbani N, Kazi TG, Arain BM, Jamali MK, Afridi HI, Sarfraz RA (2006) Talanta 70:307–314

    Article  CAS  Google Scholar 

  21. Wang L, Li D, Bao LCh, You JY, Wang ZM, Shi YH, Zhang HQ (2008) Ultrason Sonochem 15:738–746

    Article  CAS  Google Scholar 

  22. Wang L, Wang ZM, Zhang HH, Li XY, Zhang HQ (2009) Anal Chim Acta 647:72–77

    Article  CAS  Google Scholar 

  23. Wang L, Wang ZM, Li XY, Zhang HH, Zhou X, Zhang HQ (2010) Chromatographia 71:455–499

    Article  CAS  Google Scholar 

  24. Wei SG, Zhang HH, Wang YQ, Wang L, Li XY, Wang YH, Zhang HQ, Xu X, Shi YH (2011) J Chromatogr A 1218:4599–4605

    Article  CAS  Google Scholar 

  25. Zhang HH, Shi YH, Wei SG, Wang YH, Zhang HQ (2011) Talanta 85:1081–1087

    Article  CAS  Google Scholar 

  26. Lin JH, Ku YR, Lin YT, TENG SF, WEN KC, LIAO CH (2000) J Food Drug Anal 8:278–282

    CAS  Google Scholar 

  27. Leong YW, Harrison L (1999) Phytochemistry 50:849–857

    Article  CAS  Google Scholar 

  28. Liang QL, Dai CC, Wu QN, Gao Y, Jiang JH (2008) Chin Tradit Herb Drugs 39:1779–1781

    CAS  Google Scholar 

  29. Jiang Y, Jin YR, Zhang CZ, Yin ZY, Li XW, Wang HN (2010) J Jilin Univ Sci Ed 48:868–870

    CAS  Google Scholar 

  30. The State Pharmacopoeia Commission of P. R. China (2010) The pharmacopoeia of People’s Republic of China 2010. Chemical Industry Publishing House, Beijing, p 81

  31. Mason TJ, Paniwnyk L, Lorimer JP (1996) Ultrason Sonochem 3:253–260

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Chemistry, Jilin University, Changchun, 130012, People’s Republic of China

    Peixu Zhang, Yongri Jin, Hua Yao, Hanqi Zhang, Aimin Yu & Xuwen Li

  2. Department of Neurology, The First Hospital of Jilin University, Changchun, 130012, People’s Republic of China

    Jiafeng Chen

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  1. Peixu Zhang
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  2. Yongri Jin
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  3. Jiafeng Chen
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  4. Hua Yao
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  5. Hanqi Zhang
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  6. Aimin Yu
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  7. Xuwen Li
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Correspondence to Xuwen Li.

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Zhang, P., Jin, Y., Chen, J. et al. Ultrasonic-Assisted Nebulization Extraction Coupled with SPE and HPLC for the Determination of Triterpenoids in Root of Euphorbia pekinensis Rupr.. Chromatographia 76, 967–974 (2013). https://doi.org/10.1007/s10337-013-2503-x

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  • DOI: https://doi.org/10.1007/s10337-013-2503-x

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