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The Combined Effect of the Organic Modifier Content and pH of the Mobile Phase on the Chromatographic Behavior of Some Arylpropionic and Arylacetic Acids to Optimize Their Liquid Chromatographic Determinations

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Abstract

The equation that express the combined effect of the organic modifier content and mobile phase pH has been used to predict the chromatographic behavior of flurbiprofen, ibuprofen, diclofenic acid, ketoprofen, fenoprofen, naproxen, bezafibric acid and clofibric acid. Correlations between the retention factors of the solutes and pH of the mobile phase were determined from a set of 40 chromatograms for each compound at four different levels of acetonitrile composition (40, 45, 50 and 55 %, v/v), and at ten pH values in each acetonitrile–water mixtures. In this way, a complete description of the retention behavior of each solute in the space defined by the pH and organic modifier percentages variables was obtained. The optimized chromatographic condition was applied to quantitative determination of flurbiprofen, ibuprofen, and diclofenic acid in pharmaceutical dosage forms.

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Authors and Affiliations

  1. Experimental and Observational Student Practice and Research Center, Süleyman Demirel University, Isparta, Turkey

    Hale Secilmis-Canbay

  2. Department of Chemistry, Faculty of Science and Literature, Süleyman Demirel University, 32260, Isparta, Turkey

    Ebru Cubuk-Demiralay & Guleren Alsancak

  3. Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06100, Ankara, Tandogan, Turkey

    Sibel A. Ozkan

Authors
  1. Hale Secilmis-Canbay
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  2. Ebru Cubuk-Demiralay
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  3. Guleren Alsancak
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  4. Sibel A. Ozkan
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Correspondence to Sibel A. Ozkan.

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Secilmis-Canbay, H., Cubuk-Demiralay, E., Alsancak, G. et al. The Combined Effect of the Organic Modifier Content and pH of the Mobile Phase on the Chromatographic Behavior of Some Arylpropionic and Arylacetic Acids to Optimize Their Liquid Chromatographic Determinations. Chromatographia 75, 711–720 (2012). https://doi.org/10.1007/s10337-012-2255-z

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  • DOI: https://doi.org/10.1007/s10337-012-2255-z

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