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Simultaneous Determination of High-Density Lipoprotein, Very Low-Density Lipoprotein and Low-Density Lipoprotein Subclass in Human Serum by Microchip CE

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Abstract

Lipoproteins, especially high-density lipoproteins (HDL), very low-density lipoproteins (VLDL) and small, dense low-density lipoprotein (sdLDL), are believed to play an important role in the development of atherosclerosis. In this work, a simple, selective and sensitive method for the simultaneous monitoring of these lipoproteins in human serum using microchip capillary electrophoresis was developed. Gold nanoparticles were used as an additive to the running buffer to obtain the absolute separation of the lipoproteins. Under optimised conditions, the linear ranges of large buoyant low-density lipoproteins, sdLDL, VLDL and HDL were 10–800, 10–800, 40–1,000 and 20–800 μg L−1, and their limits of detection were 5, 5, 15 and 8 μg L−1, respectively. The intraassay and interassay relative standard deviation of lipoprotein peak areas were in the range of 3.8–7.4%. For practical application, variations in the serum lipoprotein of coronary heart disease patients were monitored by microchip-based CE. The results showed that the method was applicable for routine clinical use and allowed the rapid detection of different lipoprotein classes as well as their subclasses, thus greatly improving the analysis of atherosclerotic risk factors.

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Acknowledgments

This work was supported by grants from Development Program of Clinical Medical College of Yangzhou University (yzucms201041), Society Development Foundation of Jiang Su Province (BE 2010679) and the Key Subject of Jiangsu Province (XK200723).

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Authors and Affiliations

  1. Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China

    Hua Wang, Chongxu Han, Daxin Wang, Li Cao & Guangzhou Wang

  2. Affiliated Hospital of Nantong University, 20 Xi Si Road, 226001, Nantong, Jiangsu, China

    Huimin Wang

  3. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, 865 Chang Ning Road, 200050, Shanghai, China

    Qinghui Jin

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  1. Hua Wang
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  2. Chongxu Han
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  3. Huimin Wang
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  4. Qinghui Jin
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  5. Daxin Wang
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  6. Li Cao
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  7. Guangzhou Wang
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Correspondence to Hua Wang.

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Wang, H., Han, C., Wang, H. et al. Simultaneous Determination of High-Density Lipoprotein, Very Low-Density Lipoprotein and Low-Density Lipoprotein Subclass in Human Serum by Microchip CE. Chromatographia 74, 799–805 (2011). https://doi.org/10.1007/s10337-011-2147-7

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  • DOI: https://doi.org/10.1007/s10337-011-2147-7

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