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Monolithic Silica for Fast HPLC: Current Success and Promising Future

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Abstract

As an approach for fast HPLC, monolithic silica has proven to be highly effective. It is especially successful for routinely obtaining fast isocratic HPLC analyses of small drug molecules. The low cost of monolithic compared with other approaches, such as UPLC, makes it more convenient for everyday application. It is also the more developed and widely applied technique compared with superficially porous particles. It offers the possibility for gaining high plate numbers through column coupling, but not at the expense of run time if a proper flow program is subsequently applied. Good precision and batch reproducibility are now achieved with commercially available monolithic silica columns. The application of monolithic silica columns is already well developed in various fields. It invades the field of bio-analysis and proteomics. Hundreds of analytical methods have already been successfully transferred to or developed on monolithic silica columns. An updated strategy based on Snyder’s method for rapid method development using monolithic column has been provided. However, more is still to be expected from monolithic silica in term of chemistry variation, application, and instrument compatibility. The future of monolithic silica is promising when considering the high demand for fast chromatographic analysis.

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Acknowledgments

The author of this manuscript is grateful to Dr. Lutz Preu, Daphny Kaminski and Lukas Kaminski for critically reading the manuscript. Especially Lukas Kaminski contributed with very valuable ideas about the comparison of monolithic columns to alternatives. Dr. El Deeb is a research fellow of the Alexander von Humboldt Foundation at the Institute of Pharmacy of the Freie Universität Berlin and thanks them for kind support.

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  1. Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany

    Sami El Deeb

  2. Department of Pharmaceutical Chemistry, Al-Azhar University-Gaza, Gaza, Palestinian Territories

    Sami El Deeb

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El Deeb, S. Monolithic Silica for Fast HPLC: Current Success and Promising Future. Chromatographia 74, 681–691 (2011). https://doi.org/10.1007/s10337-011-2133-0

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