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Inexpensive Green Method for Diclofenac Assay Utilizing Sequential Injection Chromatography

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Abstract

Sequential injection chromatography (SIC) is a recent inexpensive miniaturized separation technique. The current manuscript reported the development of a new SIC method for the assay of diclofenac in pharmaceutical formulations. Chemometrics was exploited for the first time to optimize SIC methodology. The 23 full-factorial design was adopted to screen the effect of mobile phase composition, sample volume, and flow rate on retention time and peak height. The optimum chromatographic conditions were: C18 monolithic column (25 × 4.6 mm); mobile phase composition methanol:water (50:50, v/v), sample volume 60 μL, flow rate 40 μL s−1. The detection was carried out using a miniaturized fiber optic spectrometer set at 284 nm. Satisfactorily, separation was achieved in 0.92 min with the number of theoretical plates of >180,000 and peak symmetry 1.21. The method enjoyed good precision with relative standard deviation values for intra- and inter-day precision of 1.47 and 2.15%, respectively. The use of large sample volume rendered the method sensitive with the limits of detection and quantification of 0.32 and 1.07 μg mL−1, respectively. The remarkable advantages of the method are high rapidity (sample frequency 23 sample h−1) and reagent saving (total consumed volume 3.0 mL) and thus better safety for the environment.

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Acknowledgments

The authors express their gratitude to the financial support from the Deanship of Scientific Research, King Faisal University, Saudi Arabia, Award #10090. Thanks also are due to the Department of Chemistry, College of Science, King Faisal University for allowing the authors to conduct this work.

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Authors and Affiliations

  1. Department of Chemistry, College of Science, King Faisal University, PO Box 400, Hofuf, 31982, Saudi Arabia

    Abubakr M. Idris, Rafea E.E. Elgorashe, Fahad N. Assubaie & Ahmed O. Alnajjar

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  1. Abubakr M. Idris
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  2. Rafea E.E. Elgorashe
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  3. Fahad N. Assubaie
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  4. Ahmed O. Alnajjar
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Correspondence to Abubakr M. Idris.

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Idris, A.M., Elgorashe, R.E., Assubaie, F.N. et al. Inexpensive Green Method for Diclofenac Assay Utilizing Sequential Injection Chromatography. Chromatographia 73, 431–437 (2011). https://doi.org/10.1007/s10337-011-1936-3

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  • DOI: https://doi.org/10.1007/s10337-011-1936-3

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