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Determination of Propylene Glycol in Low Volume Plasma and Urine Samples of Neonates by LC with Photodiode Array Detection

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Abstract

In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25–50 mg L−1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15–46 mg L−1) and urine samples (20–175 mg L−1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates.

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Acknowledgments

The clinical research of Karel Allegaert is supported by the Fund for Scientific Research, Flanders (Belgium) (F.W.O. Vlaanderen) by a Fundamental Clinical Investigatorship (1800209 N), and of Aida Kulo by a JoinEU-SEE scholarship (2009–2010). We would like to thank Sonia Demarsin for her technical assistance.

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Author notes

  1. Aida Kulo

    Present address: Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71000, Sarajevo, Bosnia and Herzegovina

Authors and Affiliations

  1. Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium

    Aida Kulo, Jan de Hoon & Rene Verbesselt

  2. Neonatal Intensive Care Unit, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium

    Karel Allegaert

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  1. Aida Kulo
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  2. Karel Allegaert
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  3. Jan de Hoon
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  4. Rene Verbesselt
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Correspondence to Aida Kulo.

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Kulo, A., Allegaert, K., de Hoon, J. et al. Determination of Propylene Glycol in Low Volume Plasma and Urine Samples of Neonates by LC with Photodiode Array Detection. Chromatographia 73, 463–470 (2011). https://doi.org/10.1007/s10337-011-1923-8

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  • DOI: https://doi.org/10.1007/s10337-011-1923-8

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