Abstract
Objective
To construct siRNA recombinant expression vector targeting Livin gene and observe the apoptosis induction effect of it in human colon cancer cells.
Methods
SiRNA recombinant expression vector targeting Livin gene was constructed and transfected into human colon cancer cell. The effect of siRNA recombinant expression vector was detected by RT-PCR, Western blot, MTT reduction assay and flow cytometry.
Results
It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting Livin gene was constructed successfully. Inhibition ratio of Livin siRNA at mRNA and protein levels were 30.18% and 28.88%. The growth of cancer cells was inhibited significantly and the apoptotic ratio was 13.36 ± 1.45%.
Conclusion
The siRNA recombinant expression vector targeting Livin gene has been constructed successfully. It not only can inhibit the expression of Livin gene but also can induce apoptosis in human colon cancer cells remarkably.
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References
Liu B, Han M, Wen JK, et al. Livin/ML-IAP as a new target for cancer treatment. Cancer Lett, 2007, 250: 168–176.
Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem, 2001, 276: 3238–3246.
Ashhab Y, Alian A, Polliack A, et al. Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distribution pattern. FEBS Lett, 2001, 495: 56–60.
Crnković-Mertens I, Semzow J, Hoppe-Seyler F, et al. Isoform-specific silencing of the Livin gene by RNA interference defines Livin beta as key mediator of apoptosis inhibition in HeLa cells. J Mol Med, 2006, 84: 232–240.
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Supported by grants from the National High Technology Research and Development Program of China (No. 2001AA218051) and Educational Commission of Hubei Province of China (No. 2005A304B09).
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Cai, M., Wang, G., Tao, K. et al. The apoptosis induction effect of siRNA recombinant expression vector targeting Livin gene in human colon cancer cells. Chin. -Ger. J. Clin. Oncol. 7, 344–347 (2008). https://doi.org/10.1007/s10330-008-0044-9
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DOI: https://doi.org/10.1007/s10330-008-0044-9