Abstract
Objective: To investigate the expression of Fas, Fas ligand (FasL) and CD80 on the cell surface of mouse acute myelomonocytic leukemia cell line WEHI-3 and the function of FasL. Methods: The expression of Fas, FasL and CD80 was detected on WEHI-3 cell surface by flow cytometry. Simultaneously the function of FasL was determined by Thymidine (3H-TdR) Incorporation. Results: The expression of CD80 and Fas on WEHI-3 cell surface was 5.06%±0.41% and 6.75%±2.31% (n=5) respectively, and the expression of FasL was up to 63.73%±5.23% (n=5). The apoptotic rate of YAC-1 cells was 26%±4.5%, 35%±3.2% and 43%±2.7% (n=5) respectively when WEHI-3 (effector cell, E) and Fas+ YAC-1 cells (target cell, T) were cultured in the ratio of 3:1, 10:1 and 30:1. Conclusion: WEHI-3 cells express high FasL, low Fas and CD80, and can induce apoptosis of Fas+ YAC-1 cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Walker PR, Saas P, Dietrich PY. Role of Fas ligand (CD95L) in immune escape: the tumor cell strikes back. J Immunol, 1997, 158: 4521–4524.
Lickliter JD, Kratzke RA, Nguyen PL, et al. Fas ligand is highly expressed in acute leukemia and during the transformation of chronic myeloid leukemia to blast crisis. Exp Hematol, 1999, 27: 1519–1527.
Buzyn A, Petit F, Ostankovitch M, et al. Membranebound Fas (Apo-1/CD95) ligand on leukemic cells: A mechanism of tumor immune escape in leukemia patients. Blood, 1999, 94: 3135–3140.
Whiteway A, Corbett T, Anderson R, et al. Expression of co-stimulatory molecules on acute myeloid leukaemia blasts may effect duration of first remission. Br J Haematol, 2003, 120: 442–451.
Bremner TA, Chatterjee D, Han Z, et al. THP-1 monocytic leukemia cells express Fas ligand constitutively and kill Fas-positive Jurkat cells. Leuk Res, 1999, 23: 865–870.
Brouwer RE, Zwinderman KH, Kluin-Nelemans HC, et al. Expression and induction of costimulatory and adhesion molecules on acute myeloid leukemic cells: implications for adoptive immunotherapy. Exp Hematol, 2000, 28: 161–168.
Furukawa Y, Kubota R, Tara M, et al. Existence of escape mutant in HTLV-I tax during the development of adult T-cell leukemia. Blood, 2001, 97: 987–993.
Mohty M, Jarrossay D, Lafage-Pochitaloff M, et al. Circulating blood dendritic cells from myeloid leukemia patients display quantitative and cytogenetic abnormalities as well as functional impairment. Blood, 2001, 98: 3750–3756.
Chen X, Woiciechowsky A, Raffegerst S, et al. Impaired expression of the CD3-zeta chain in peripheral blood T cells of patients with chronic myeloid leukaemia results in an increased susceptibility to apoptosis. Br J Haematol, 2000, 111: 817–825.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by a grant from National natural Sciences Foundation of China (30240022).
Rights and permissions
About this article
Cite this article
Li, W., Liu, L., He, W. et al. Initial study on immune escape mechanism of mouse acute myelomonocytic leukemic cell line WEHI-3. Chinese German J Clin Oncol 5, 291–293 (2006). https://doi.org/10.1007/s10330-005-0433-2
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10330-005-0433-2