Skip to main content
SpringerLink
Log in

Effects of coinfection with East Asian Passiflora virus and East Asian Passiflora distortion virus on Passiflora foetida

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

East Asian Passiflora virus (EAPV) and East Asian Passiflora distortion virus (EAPDV), members of the potyvirus genus, infect passionfruit (Passiflora edulis × P. edulis f. flavicarpa). We found EAPV and EAPDV coinfecting passionfruit in Okinawa Prefecture, Japan and here investigated the effects of coinfection with those viruses on P. foetida, a wild passionfruit. Coinfection with EAPV and EAPDV resulted in different symptoms and viral titers in P. foetida. Mixed inoculation (EAPV + EAPDV) and inoculation with EAPV and 6 days later with EAPDV (EAPV → EAPDV) caused the most severe leaf symptoms at 28 dpi, whereas inoculation with EAPDV then EAPV (EAPDV → EAPV) induced milder symptoms. Single infection with EAPDV caused the mildest symptoms. Quantitative analysis revealed that the log10 of EAPDV titer in EAPDV → EAPV, EAPV → EAPDV, and D + A, was 1.85-, 1.52-, and 1.56-fold higher, respectively, than after a single infection with EAPDV. EAPV titer was always lower than the EAPDV titer. In short, EAPV induced EAPDV propagation, and P. foetida is a potential reservoir for EAPV and EAPDV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Ajitomi A, Miyagi S, Inada T, Ooshiro A, Yamashiro M, Matsumura M, Ahagon N, Oshiro K, Nagahama R, Medoruma K, Kajiyama T, Ooishi A, Shimoji A, Tokuyama M, Kijima K, Yamaguchi A, Hosokawa R, Teramura K, Iwai H (2018) Occurrence of virus diseases in passionfruit field in Okinawa Prefecture (abstract in Japanese). Jpn J Phytopathol 84:70

    Google Scholar 

  • Čeřovská N, Hoffmeisterová H, Pečenkova T, Moravec T, Synková H, Plchová H, Velemínsky J (2007) Transient expression of HPV16 E7 peptide (aa 44–60) and HPV16L2 peptide (aa 108–120) on chimeric potyvirus-like particles using Potato virus X-based vector. Protein Expr Purif 58:154–161

    Article  Google Scholar 

  • Cháves-Calvillo G, Contreras-Paredes CA, Mora-Macias J, Noa-Carrazana JC, Serrano-Rubio AA, Dinkova TD, Carrillo-Tripp M, Silva-Rosales L (2016) Antagonism or synergism between papaya ringspot virus and papaya mosaic virus in Carica papaya is determined by their order of infection. Virology 489:179–191

    Article  Google Scholar 

  • Davis RI, Thomas JE, McMichael LA, Dietzgen RG, Callaghan B, James AP, Gunua TG, Rahamma S (2002) Plant virus surveys on the island of New Guinea and adjacent regions of northern Australia. Aust Plant Pathol 31:385–390

    Article  Google Scholar 

  • Fukumoto T, Nakamura M, Ohshima K, Iwai H (2012) Genetic structure and variability of East Asian Passiflora virus population in Amami-O-shima, Japan. J Phytopathol 160:404–411

    Article  CAS  Google Scholar 

  • García-Marcos A, Pacheco R, Martiáñez J, González-Jara P, Díaz-Ruíz JR, Tenllado F (2009) Transcriptional changes and oxidative stress associated with the synergistic interaction between Potato virus X and Potato virus Y and their relationship with symptom expression. Mol Plant Microbe Interact 22:1431–1444

    Article  Google Scholar 

  • González-Jara P, Tenllado F, Martínez-Garcia B, Atencio F, Barajas D, Vargas M, Díaz-Ruíz J, Díaz-Ruíz JR (2004) Host-dependent differences during synergistic infection by potyviruses with potato virus X. Mol Plant Pathol 5:29–35

    Article  Google Scholar 

  • González-Jara P, Fraile A, Canto T, Garzía-Arenal F (2009) The multiplicity of infection of a plant virus varies during colonization of its eukaryotic host. J Virol 87:7487–7494

    Article  Google Scholar 

  • Iwai H (2017) Studies on diagnosis and genomic analysis of plant viral diseases in southern Japan. J Gen Plant Pathol 83:414–418

    Article  Google Scholar 

  • Iwai H, Ohmori T, Kurokawa Y, Muta T, Arai K (1996) New record of passionfruit woodiness virus in Japan. Ann Phytopathol Soc Jpn 62:459–465

    Article  Google Scholar 

  • Iwai H, Yamashita Y, Nishi N, Nakamura M (2006) The potyvirus associated with the dappled fruit of Passiflora edulis in Kagoshima prefecture, Japan is the third strain of the proposed new species East Asian Passiflora virus (EAPV) phylogenetically distinguished from strains of Passionfruit woodiness virus. Arch Virol 151:811–818

    Article  CAS  Google Scholar 

  • Kokkinos CD, Clark CA (2006) Interactions among sweet potato chlorotic stunt virus and different potyviruses and potyvirus strains infecting sweet potato in the United States. Plant Dis 90:1347–1352

    Article  CAS  Google Scholar 

  • Leggat FW, Teakle DS (1975) Passiflora foetida, a widespread host of passionfruit woodiness virus in Queensland. Aust Plant Pathol Soc Newslett 4:22–23

    Article  Google Scholar 

  • Liang Z, Dickison V, Singh M, Xiong X, Nie X (2016) Studies of tomato plants in response to infections with PVX and different PVY isolates reveal a remarkable PVX-PVYNTN synergism and diverse expression profiles of genes involved in different pathways. Eur J Plant Pathol 144:55–71

    Article  CAS  Google Scholar 

  • Nie X, Singh M (2013) Response of potato, tobacco and Physalis floridana plants to mixed infection with PVX, PVYNTN and PVYO strains. Can J Plant Pathol 35:390–401

    Article  CAS  Google Scholar 

  • Parry JN, Davis RI, Thomas JE (2004) Passiflora virus Y, a novel virus infecting Passiflora spp. in Australia and the Indonesian Province of Papua. Aust Plant Pathol 33:423–427

    Article  Google Scholar 

  • Riska, Sato Y, Inudo K, Nakamura M, Fukumoto T, Takushi T, Fuji S, Iwai H (2019) East Asian Passiflora distortion virus: a new Potyvirus species causing deformation of passionfruit in Japan. J Gen Plant Pathol 85:221–231

    Article  Google Scholar 

  • Syller J (2012) Facilitative and antagonistic interactions between plant viruses in mixed infections. Mol Plant Pathol 13:204–216

    Article  Google Scholar 

  • Tatineni S, Graybosch RA, Hein GL, Wegulo SN, French R (2010) Wheat cultivar-specific disease synergism and alteration of virus accumulation during co-infection with Wheat streak mosaic virus and Triticum mosaic virus. Phytopathology 100:230–238

    Article  CAS  Google Scholar 

  • Tatineni S, Kovacs F, French R (2014) Wheat streak mosaic virus infects systemically despite extensive coat protein deletions: identification of virion assembly and cell-to-cell movement determinants. J Virol 88:1366–1380

    Article  Google Scholar 

  • Vance VB (1991) Replication of potato virus X is altered in coinfections with potato virus Y. Virology 206:583–590

    Article  Google Scholar 

  • Vance VB, Berger PH, Carrington JC, Hunt AG, Shi XM (1995) 5′-Proximal potyviral sequences mediate potato virus X/potyviral synergistic disease in transgenic tobacco. Virology 206:583–590

    Article  CAS  Google Scholar 

  • Yonaha T, Tamori M, Yamanoha S, Nakasone T (1979) Studies on passionfruit virus diseases in Okinawa: 1. Cucumber mosaic virus isolated from diseased Passiflora edulis and Passiflora foetida plants (in Japanese). Sci Bull Coll Agric Univ Ryukyus 26:29–38

    Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge support from the JSPS KAKENHI Grant Number 16K07620 and from World Bank Funds regulated by the Indonesian Agency of Agriculture Research and Development (IAARD).

Author information

Authors and Affiliations

  1. The United Graduate School of Agricultural Science, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan

    Riska, Masayuki Nakamura & Hisashi Iwai

  2. Faculty of Agriculture, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan

    Riska, Masayuki Nakamura & Hisashi Iwai

  3. Indonesian Tropical Fruits Research Institute, Indonesian Agency of Agriculture Research and Development, Jl. Raya, Solok-Aripan, Km 8, PO. BOX 5, Solok, West Sumatra, 27356, Indonesia

    Riska

Authors
  1. Riska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masayuki Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hisashi Iwai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hisashi Iwai.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Riska, Nakamura, M. & Iwai, H. Effects of coinfection with East Asian Passiflora virus and East Asian Passiflora distortion virus on Passiflora foetida. J Gen Plant Pathol 86, 211–218 (2020). https://doi.org/10.1007/s10327-020-00913-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-020-00913-7

Keywords

Search

Navigation