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Genetic and phenotypic characterization of bacterial strains isolated in Japan that resemble Erwinia rhapontici and E. persicinus

  • Bacterial and Phytoplasma Diseases
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Abstract

The taxonomic identity of Japanese isolates of bacteria with physiological and biochemical characteristics similar to Erwinia rhapontici or E. persicinus was clarified using genetic analyses, pathogenicity tests and phenotypic characterization. In a phylogenetic analysis based on 16S rRNA and housekeeping genes gyrB, rpoD, and recA, the strains formed two independent clusters, one for E. rhapontici and another for E. persicinus, each including the type strain. The E. rhapontici cluster had three subclusters: pink-pigmented strains isolated in Japan, the type strain, and nonpigmented strains isolated from wasabi. rep-PCR banding patterns confirmed this grouping. The ability to cause rot on plants and the rot severity depended on the strain, though virulence of E. rhapontici strains isolated in Japan tended to be relatively weaker than that of the others. Pink pigment formation by E. rhapontici and E. persicinus strains was abundant on onion, Welsh onion and garlic, but E. rhapontici strains isolated from wasabi did not produce pink pigment on any plants or media tested. Thus, the unknown strains from Japan that produced pink pigment were E. rhapontici or E. persicinus. Despite being nonpigmented, E. rhapontici strains from wasabi were genetically closely related to pink-pigmented E. rhapontici strains. Based on the rpoD sequence, two specific primer sets were newly designed to discriminate E. rhapontici and E. persicinus.

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References

  • Born Y, Remus-Emsermann MNP, Bieri M, Kamber T, Piel J, Pelludat C (2016) Fe2+ chelator proferrorosamine A: a gene cluster of Erwinia rhapontici P45 involved in its synthesis and its impact on growth of Erwinia amylovora CFBP1430. Microbiol 162:236–245

    Article  CAS  Google Scholar 

  • Bradbury JF (1977) Erwinia rhapontici. IMI descriptions of fungi and bacteria, 1977, no. 56, pp sheet 555. CABI, Wallingford, UK

  • Dye DW (1969) A taxonomic study of the genus Erwinia II. The “carotovora” group. New Zealand J Sci 12:81–97

    Google Scholar 

  • Feistner G, Korth H, Ko H, Pulverer G, Budzikiewicz H (1983) Ferrorosamine A from Erwinia rhapontici. Curr Microbiol 8:239–243

    Article  CAS  Google Scholar 

  • Feistner GJ, Mavridis A, Rudolph K (1997) Proferrorosamines and phytopathogenicity in Erwinia spp. Biometals 10:1–10

    Article  CAS  Google Scholar 

  • Gálvez L, Gil-Serna J, García-Díaz M, Palmero D (2015) First report of a garlic bulb rot caused by Erwinia persicina in Europe. Plant Dis 99:723

    Article  Google Scholar 

  • González AJ, Tello JC, Cara M (2005) First report of Erwinia persicina from Phaseolus vulgaris in Spain. Plant Dis 89:109

    Article  Google Scholar 

  • González AJ, Tello JC, Rodicio MR (2007) Erwinia persicina causing chlorosis and necrotic spots in leaves and tendrils of Pisum sativum in southeastern Spain. Plant Dis 91:460

    Article  Google Scholar 

  • Goto M, Matsumoto K (1986) Taxonomic study on soft rot bacteria isolated from diseased rhizomes and roots of wasabi (Eutrema wasabi Maxim.). Ann Phytopathol Soc Jpn 52:69–77

    Article  Google Scholar 

  • Hao MV, Brenner DJ, Steigerwalt AG, Kosako Y, Komagata K (1990) Erwinia persicinus, a new species isolated from plants. Int J Syst Bacteriol 40:379–383

    Article  CAS  Google Scholar 

  • Hiraishi A (1995) Genetic analysis and phylogeny based on 16S ribosomal RNA through polymerase chain reaction. (in Japanese). Bull J Soc Microbial Ecol 10:31–42

    Article  Google Scholar 

  • Huang HC, Hsieh TF, Erickson RS (2003) Biology and epidemiology of Erwinia rhapontici, causal agent of pink seed and crown rot of plants. Plant Pathol Bull 12:69–76

    Google Scholar 

  • Kawase K, Takikawa Y (2008) Diversity of Pectobacterium spp. isolated from Japanese horseradish (Eutrema wasabi) and their biological control (Abstract in Japanese). Jpn J Phytopathol 74:252

    Google Scholar 

  • Kelman A (1954) The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance in a tetrazolium medium. Phytopathol 44:693–695

    Google Scholar 

  • King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44:301–307

    CAS  PubMed  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Kusumoto S, Aeny TN, Mujimu S, Ginting C, Tsuge T, Tsuyumu S, Takikawa Y (2004) Occurrence of blood disease of banana in Sumatra, Indonesia. J Gen Plant Pathol 70:45–49

    Article  Google Scholar 

  • Letal JR (1976) Crown rot of rhubarb in Alberta. Can Plant Dis Surv 56:67–68

    Google Scholar 

  • Li S, Cai H, Qing Y, Ren B, Xu H, Zhu H, Yao J (2011) Cloning and characterization of a sucrose isomerase from Erwinia rhapontici NX-5 for isomaltulose hyperproduction. Appl Biochem Biotechnol 163:52–63

    Article  CAS  Google Scholar 

  • Maeda Y, Shinohara H, Kiba A, Ohnishi K, Furuya N, Kawamura Y, Ezaki T, Vandamme P, Tsushima S, Hikichi Y (2006) Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences. Int J Syst Evol Microbiol 56:1031–1038

    Article  CAS  Google Scholar 

  • Metcalfe G (1940) Bacterium rhaponticum (Millard) Dowson, a cause of crown-rot disease of rhubarb. Ann Appl Biol 27:502–508

    Article  Google Scholar 

  • Millard WA (1924) Crown rot of rhubarb. Bull Univ Leeds Yorks Coun Agric Educ 134:1–28

    Google Scholar 

  • Nishiyama K, Ezuka A (1977) Rough-colony isolates of Pseudomonas coronafaciens var atropurpurea obtained from diseased leaves of ryegrasses (in Japanese with English summary). Ann Phytopath Soc Jpn 43:426–431

    Article  Google Scholar 

  • Nishiyama N, Makino T, Takikawa Y, Tsuyumu S, Goto M (1985) Erwinia rhapontici and Pseudomonas viridiflava isolated from various plant species (abstract in Japanese). Ann Phytopath Soc Jpn 51:55

    Google Scholar 

  • O’Hara CM, Steigerwalt AG, Hill BC, Miller JM, Brenner DJ (1998) First report of a human isolate of Erwinia persicinus. J Clin Microbiol 36:248–250

    Article  Google Scholar 

  • Ohuchi A, Ohsawa T, Nishimura J (1983) Two pathogenic bacteria, Erwinia rhapontici (Millard 1924) Burkholder 1948 and Pseudomonas marginalis pv. marginalis (Brown 1918) Stevens 1925, causing a soft rot of onion (in Japanese with English summary). Ann Phytopathol Soc Jpn 49:619–626

    Article  Google Scholar 

  • Rademaker JLW, Louws FJ, Versalovic J, de Bruijn FJ (1998) Characterization of the diversity of ecologically important microbes by rep-PCR genomic fingerprinting. In: Akkermans ADL, van Elsas JD, de Bruijn FJ (eds) Molecular microbial ecology manual, suppl 3. Kluwer, Dordrecht, pp 1–27

    Google Scholar 

  • Roberts P (1974) Erwinia rhapontici (Millard) Burkholder associated with pink grain of wheat. J Appl Bacteriol 37:353–358

    Article  CAS  Google Scholar 

  • Sellwood JE, Lelliott RA (1978) Internal browning of hyacinth caused by Erwinia rhapontici. Plant Pathol 27:120–124

    Article  Google Scholar 

  • Society of American Bacteriologists (1957) Manual of microbiological methods. McGraw-Hill, New York, p 54

    Google Scholar 

  • Stockwell VO, Hockett K, Marie C, Duffy B (2008) Pink Erwinia amylovora: colony discoloration in diagnostic isolations by co-cultured bacteria. Acta Hortic 793:539–542

    Article  Google Scholar 

  • Takahashi F, Ogiso H, Fujinaga M, Ishiyama Y, Inoue Y, Shirakawa T, Takikawa Y (2013) First report of bacterial blight of crucifers caused by Pseudomonas cannabina pv. alisalensis in Japan. J Gen Plant Pathol 79:260–269

    Article  CAS  Google Scholar 

  • Thapa SP, Cho SY, Hur JH, Lim CK (2012) Phenotypic and genetic characterization of Erwinia rhapontici isolated from diseased Asian pear fruit trees. Phytoparasitica 40:507–514

    Article  CAS  Google Scholar 

  • Tsuji M, Takikawa Y (2012) Classification of bacteria resembling to Erwinia rhapontici or Erwinia persicinus isolated from various crops in Japan (Abstract in Japanese). Jpn J Phytopathol 78:273

    Google Scholar 

  • Tsuji M, Ohta K, Tanaka K, Takikawa Y (2017) Comparison among Japanese isolates of Pseudomonas savastanoi pv. savastanoi, causal agent of olive knot disease. J Gen Plant Pathol 83:152–161

    Article  CAS  Google Scholar 

  • Waleron M, Waleron K, Podhajska AJ, Łojkowska E (2002) Genotyping of bacteria belonging to the former Erwinia genus by PCR-RFLP analysis of a recA gene fragment. Microbiol 148:583–595

    Article  CAS  Google Scholar 

  • Wilson EE, Zeitoun FM, Fredrickson DL (1967) Bacterial phloem canker, a new disease of Persian walnut trees. Phytopathol 57:618–621

    Google Scholar 

  • Woestyne MV, Bruyneel B, Mergeay M, Verstraete W (1991) The Fe2+ chelator proferrorosamine A is essential for the siderophore-mediated uptake of iron by Pseudomonas roseus fluorescens. Appl Environ Microbiol 57:949–954

    Article  Google Scholar 

  • Yamamoto S, Bouvet PJM, Harayama S (1999) Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA–DNA hybridization. Int J Syst Bacteriol 49:87–95

    Article  CAS  Google Scholar 

  • Zhang ZF, Nan ZB (2012) First report of Erwinia persicinus causing wilting of Medicago sativa sprouts in China. Plant Dis 96:454

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We sincerely thank Mr. Koichi Kawase for his cooperation in many experiments at the plant pathology laboratory of Shizuoka University.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka, 422-8529, Japan

    Mizue Tsuji & Yuichi Takikawa

  2. National Agriculture and Food Research Organization, Tohoku Agricultural Research Center, 4 Akahira Shimo-kuriyagawa, Morioka, Iwate, 020-0198, Japan

    Mizue Tsuji & Ikuo Kadota

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  1. Mizue Tsuji
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  2. Ikuo Kadota
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  3. Yuichi Takikawa
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Correspondence to Mizue Tsuji.

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Tsuji, M., Kadota, I. & Takikawa, Y. Genetic and phenotypic characterization of bacterial strains isolated in Japan that resemble Erwinia rhapontici and E. persicinus. J Gen Plant Pathol 86, 24–33 (2020). https://doi.org/10.1007/s10327-019-00873-7

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