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Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)

  • Fungal Diseases
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Abstract

Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.

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Acknowledgements

This study was partly supported by Grants-in-Aid from The Japan Society for the Promotion of Sciences (JSPS) to TA (16H02536 and 18380030) and Building of Consortia for the Development of Human Resources in Science and Technology (Innovation Advancement Organization, TUAT) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank ASKA Pharmaceutical (Tokyo, Japan) for providing Driselase. We are grateful to Dr. Yoshimiki Amemiya (Chiba University, Matsudo, Japan) for providing a fungal isolate and Dr. Takashi Tsuge (Nagoya University, Nagoya, Japan) for providing plasmid DNA.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan

    Yu Ayukawa, Ken Komatsu & Tsutomu Arie

  2. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo, 183-8509, Japan

    Yu Ayukawa

  3. Institute of Global Innovation Research (GIR), Tokyo University of Agriculture and Technology (TUAT), Fuchu, 183-8509, Japan

    Ken Komatsu & Tsutomu Arie

  4. Department of Biology, Faculty of Science, Okayama University, Okayama, 700-8530, Japan

    Masatoki Taga

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  1. Yu Ayukawa
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  2. Ken Komatsu
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  4. Tsutomu Arie
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Correspondence to Tsutomu Arie.

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Ayukawa, Y., Komatsu, K., Taga, M. et al. Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM). J Gen Plant Pathol 84, 254–261 (2018). https://doi.org/10.1007/s10327-018-0784-5

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  • DOI: https://doi.org/10.1007/s10327-018-0784-5

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