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DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease

  • Viral and Viroid Diseases
  • Published:
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Abstract

A method to quantify resting spores of Olpidium virulentus, fungal vector of Mirafiori lettuce big-vein virus, which causes lettuce big-vein disease, in lettuce roots was developed using a DAS-ELISA with a polyclonal antibody against the resting spores and used to evaluate the relationship between the resting spore density in soil and disease severity. When lettuce seedlings were grown on soils containing a powder made from diseased roots with a known number of resting spores, symptoms developed from ca. 102 spores/g of soil or more. This simple method can be used in soil diagnosis and risk assessment of the disease.

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Acknowledgments

We are very grateful to Toshie Nakai and Tomoko Ogawa (Western Region Agricultural Research Center) for technical assistance.

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Authors and Affiliations

  1. Western Region Agricultural Research Center, National Agriculture and Food Research Organization, Fukuyama, Hiroshima, 721-8514, Japan

    Koji Nomiyama, Hiroyuki Sekiguchi, Keisuke Tomioka, Hideki Osaki & Toshiaki Takehara

  2. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 812-8581, Japan

    Koji Nomiyama, Minoru Takeshita, Naruto Furuya & Kenichi Tsuchiya

  3. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, Koshi, Kumamoto, 861-1192, Japan

    Takahide Sasaya

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  1. Koji Nomiyama
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  2. Takahide Sasaya
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  3. Hiroyuki Sekiguchi
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  4. Keisuke Tomioka
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  5. Hideki Osaki
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  6. Minoru Takeshita
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  8. Naruto Furuya
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  9. Kenichi Tsuchiya
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Correspondence to Koji Nomiyama.

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Nomiyama, K., Sasaya, T., Sekiguchi, H. et al. DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease. J Gen Plant Pathol 81, 243–248 (2015). https://doi.org/10.1007/s10327-015-0586-y

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  • DOI: https://doi.org/10.1007/s10327-015-0586-y

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