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Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae

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Abstract

The rDNA-ITS sequences of ten single-sporangium isolates of Olpidium virulentus (a noncrucifer strain of Olpidium brassicae), which transmits Mirafiori lettuce big-vein virus (MLBVV) and tobacco stunt virus (TStV), were compared with those of six single-sporangium isolates of O. brassicae. The sequence similarity within isolates of O. virulentus or O. brassicae was almost identical (98.5%–100.0%), but was low between the two species (79.7%–81.8%). In a phylogenetic analysis of the rDNA-ITS region, O. virulentus and O. brassicae fell into two distinct clusters, indicating that O. virulentus, a vector of MLBVV and TStV, is a distinct species rather than a strain of O. brassicae.

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  1. Takahide Sasaya

    Present address: Policy Planning and Evaluation Division, Minister's Secretariat, MAFF, Tokyo, Japan

Authors and Affiliations

  1. National Agricultural Research Center for Western Region, Zentsuji, Kagawa, 765-8508, Japan

    Takahide Sasaya

  2. Kunisada Plant Breeding Station, Kaneko Seeds, Gunma, Japan

    Hiroki Koganezawa

Authors
  1. Takahide Sasaya
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  2. Hiroki Koganezawa
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Correspondence to Takahide Sasaya.

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Sasaya, T., Koganezawa, H. Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae. J Gen Plant Pathol 72, 20–25 (2006). https://doi.org/10.1007/s10327-005-0239-7

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  • DOI: https://doi.org/10.1007/s10327-005-0239-7

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