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Simultaneous detection by multiplex PCR of the high-temperature-growing Pythium species: P. aphanidermatum, P. helicoides and P. myriotylum

  • Fungal Diseases
  • Published:
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Abstract

The objective of this study was to develop a multiplex PCR detection method for the high-temperature-growing pathogens Pythium aphanidermatum, P. helicoides and P. myriotylum. Species-specific primer pairs were designed that targeted the rDNA ITS regions. The multiplex PCR was constructed with a universal primer pair for eukaryotes directed at the 18S rDNA as a positive control, in addition to the three species-specific primer pairs. When the multiplex PCR was applied to naturally infested soils, the expected species were reliably identified, suggesting that the method is suitable for the detection of the three Pythium pathogens in environmental samples.

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Authors and Affiliations

  1. River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Yasushi Ishiguro, Takahiro Asano, Kayoko Otsubo & Koji Kageyama

  2. Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Haruhisa Suga

Authors
  1. Yasushi Ishiguro
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  2. Takahiro Asano
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  3. Kayoko Otsubo
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  4. Haruhisa Suga
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  5. Koji Kageyama
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Correspondence to Yasushi Ishiguro.

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Ishiguro, Y., Asano, T., Otsubo, K. et al. Simultaneous detection by multiplex PCR of the high-temperature-growing Pythium species: P. aphanidermatum, P. helicoides and P. myriotylum . J Gen Plant Pathol 79, 350–358 (2013). https://doi.org/10.1007/s10327-013-0466-2

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  • DOI: https://doi.org/10.1007/s10327-013-0466-2

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