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Journal of General Plant Pathology

, Volume 79, Issue 3, pp 214–216 | Cite as

First report of tomato chlorotic dwarf viroid isolated from symptomless petunia plants (Petunia spp.) in Japan

  • Takuya Shiraishi
  • Kensaku Maejima
  • Ken Komatsu
  • Masayoshi Hashimoto
  • Yukari Okano
  • Yugo Kitazawa
  • Yasuyuki Yamaji
  • Shigetou NambaEmail author
Disease Note

Abstract

A viroid was detected for the first time in symptomless petunia plants (Petunia spp.) and identified as Tomato chlorotic dwarf viroid (TCDVd) based on an analysis of the complete genomic sequence. These petunia plants are a likely source of inoculum for tomato or potato plants because TCDVd induces severe symptoms on these plants. The genomic sequence of this petunia isolate from Japan shared 100 % identity with petunia isolates from the Netherlands and United Kingdom and a tomato isolate from Japan. Phylogenetic analysis showed that all petunia isolates and the tomato isolate from Japan formed a monophyletic clade.

Keywords

Petunia Petunia spp. Potato spindle tuber viroid (PSTVd) Tomato chlorotic dwarf viroid (TCDVd) 

Notes

Acknowledgments

This work was supported by Grants-in-Aids for Scientific Research from the Japan Society for the Promotion of Science.

References

  1. Behjatnia SAA, Dry IB, Krake LR, Condé BD, Connelly MI, Randles JW, Rezaian MA (1996) New potato spindle tuber viroid and tomato leaf curl geminivirus strains from a wild Solanum sp. Phytopathology 86:880–886CrossRefGoogle Scholar
  2. Bostan H, Nie X, Singh RP (2004) An RT-PCR primer pair for the detection of Pospiviroid and its application in surveying ornamental plants for viroids. J Virol Methods 116:189–193PubMedCrossRefGoogle Scholar
  3. Ding B (2009) The biology of viroid–host interactions. Annu Rev Phytopathol 47:105–131PubMedCrossRefGoogle Scholar
  4. Flores R, Hernández C, Martínez de Alba AE, Daròs JA, Di Serio F (2005) Viroids and viroid–host interactions. Annu Rev Phytopathol 43:117–139PubMedCrossRefGoogle Scholar
  5. Hosokawa M, Matsushita Y, Uchida H, Yazawa S (2006) Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue. J Virol Methods 131:28–33PubMedCrossRefGoogle Scholar
  6. James T, Mulholland V, Jeffries C, Chard J (2008) First report of Tomato chlorotic dwarf viroid infecting commercial petunia stocks in the United Kingdom. Plant Pathol 57:400CrossRefGoogle Scholar
  7. Matsushita Y, Kanda A, Usugi T, Tsuda S (2008) First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan. J Gen Plant Pathol 74:182–184CrossRefGoogle Scholar
  8. Matsushita Y, Usugi T, Tsuda S (2009) Host range and properties of Tomato chlorotic dwarf viroid. Eur J Plant Pathol 124:349–352CrossRefGoogle Scholar
  9. Matsushita Y, Usugi T, Tsuda S (2010) Development of a multiplex RT-PCR detection and identification system for Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. Eur J Plant Pathol 128:165–170CrossRefGoogle Scholar
  10. Matsushita Y, Usugi T, Tsuda S (2011) Distribution of tomato chlorotic dwarf viroid in floral organs of tomato. Eur J Plant Pathol 130:441–447CrossRefGoogle Scholar
  11. Matsuura S (2012) Transmission and disease control of Tomato chlorotic dwarf viroid (in Japanese). Plant Protect 66:232–235Google Scholar
  12. Mertelik J, Kloudova K, Cervena G, Necekalova J, Mikulkova H, Levkanicova Z, Dedic P, Ptacek J (2010) First report of Potato spindle tuber viroid (PSTVd) in Brugmansia spp., Solanum jasminoides, Solanum muricatum and Petunia spp. in the Czech Republic. Plant Pathol 59:392CrossRefGoogle Scholar
  13. Owens RA (2007) Potato spindle tuber viroid: the simplicity paradox resolved? Mol Plant Pathol 8:549–560PubMedCrossRefGoogle Scholar
  14. Tsushima T, Murakami S, Ito H, He YH, Charith Raj AP, Sano T (2011) Molecular characterization of Potato spindle tuber viroid in dahlia. J Gen Plant Pathol 77:253–256CrossRefGoogle Scholar
  15. Verhoeven JTJ, Jansen CCC, Werkman AW, Roenhorst JW (2007) First report of Tomato chlorotic dwarf viroid in Petunia hybrida from the United States of America. Plant Dis 91:324CrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan 2013

Authors and Affiliations

  • Takuya Shiraishi
    • 1
  • Kensaku Maejima
    • 1
  • Ken Komatsu
    • 1
  • Masayoshi Hashimoto
    • 1
  • Yukari Okano
    • 1
  • Yugo Kitazawa
    • 1
  • Yasuyuki Yamaji
    • 1
  • Shigetou Namba
    • 1
    Email author
  1. 1.Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan

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