Abstract
Fifty wheat varieties along with Jupateco-73 and Morocco were studied for the expression of leaf tip necrosis (LTN), a trait linked with the durable rust resistance gene pair Lr34/Yr18. LTN was frequent (i.e., ≥6) in nine replications of a field experiment over 3 years in 17 genotypes, and the varieties were considered positive for LTN. In molecular analyses of these varieties, having relative severity values up to 78 for yellow rust and 45 for leaf rust, the 150-bp Lr34/Yr18-linked allele was consistently amplified. Expression of LTN in six of nine replications is an appropriate threshold for predicting the presence of Lr34/Yr18 gene pair, and genotypes can be selected using this trait.
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Afzal SN, Haque MI, Ahmedani MS, Rauf CA, Munir M, Firdous SS, Rattu AR, Ahmad I (2008) Impact of stripe rust on kernel weight of wheat varieties sown in rainfed areas of Pakistan. Pak J Bot 40:923–929
Fayyaz M, Rattu AR, Ahmad I, Akhtar MA, Hakro AA, Kazi AM (2008) Current status of the occurrence and distribution of (Puccinia triticina) wheat leaf rust virulence in Pakistan. Pak J Bot 40:887–895
Hare RA, McIntosh RA (1979) Genetic and cytogenetic studies of durable adult-plant resistances in ‘‘Hope’’ and related cultivars to wheat rusts. Z Pflanzenzüchtung 83:350–367
Kolmer JA, Singh RP, Garvin DF, Viccars L, William HM, Huerta-Espino J, Ogbonnaya FC, Raman H, Orford S, Bariana HS, Lagudah ES (2008) Analysis of theLr34/Yr18 rust resistance region in wheat germplasm. Crop Sci 48:1841–1852
Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363
Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Spielmeyer W (2006) Molecular genetic characterisation of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30
Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, Victoria
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500
Rehman RU (2006) Virulence analyses of Puccinia triticina population and gene postulation in current genotypes of wheat against leaf rust in Pakistan. University of Arid Agriculture (Rawalpindi), Pakistan, PhD thesis
Shah SJA, Imtiaz M, Hussain S (2010) Phenotypic and molecular characterization of wheat for slow rusting resistance against Puccinia striiformis Westend. f. sp. tritici. J Phytopathol 158:393–402
Singh RP (1992) Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in wheat. Crop Sci 32:874–878
Singh RP (1993) Genetic association of gene Bdv1 for barley yellow dwarf virus with genes Lr34 and Yr18 for adult plant resistance to rusts in bread wheat. Plant Dis 77:1103–1106
Singh RP, Huerta-Espino J, William M (2001) Slow rusting genes based resistance to leaf and yellow rusts in wheat: Genetics and breeding at CIMMYT. In: Proceedings of the 10th Assembly of the Wheat Breeding Society of Australia, Mildura, Australia, 16–21 September 2001, pp 103–108
Singh RP, Rajaram S (1994) Genetics of adult plant resistance to stripe rust in ten spring bread wheats. Euphytica 72:1–7
Singh RP, Chen WQ, He ZH (1999) Leaf rust resistance of spring, facultative, and winter wheat cultivars from China. Plant Dis 83:644–651
Singh RP, William HM, Huerta-Espino J, Rosewarne G (2005) Wheat rust in Asia: meeting the challenges with old and new technologies. Proceedings of the 4th International Crop Science Congress, Brisbane, Australia, 26 September–1 October 2004 (http://www.cropscience.org.au/icsc2004/symposia/3/7/141_singhrp.htm)
Wamishe YA, Milus EA (2004) Genes for adult-plant resistance to leaf rust in soft red winter wheat. Plant Dis 88:1107–1114
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Shah, S.J.A., Hussain, S., Ahmad, M. et al. Using leaf tip necrosis as a phenotypic marker to predict the presence of durable rust resistance gene pair Lr34/Yr18 in wheat. J Gen Plant Pathol 77, 174–177 (2011). https://doi.org/10.1007/s10327-011-0301-6
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DOI: https://doi.org/10.1007/s10327-011-0301-6