Abstract
In November 2008 in Himachal Pradesh and Chandigarh regions in India, toon trees and periwinkles were observed to have formed short internodes, small leaves and witches’-broom symptoms, typical of phytoplasma infection. The symptomatic toon and periwinkle samples were tested with universal PCR tests, and the 16S rRNA, rplB-rpsC, secA and secY genes were sequenced. The causal agents belonged to subgroup 16SrI-B of ‘Candidatus Phytoplasma asteris’, based on 16S rDNA, ribosomal protein gene, secA and secY phylogenetic analysis.
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Acknowledgments
The authors thank the Director of the Institute of Himalayan Bioresource Technology (CSIR), Palampur, India for providing necessary facilities to do this work. OFA thanks CCSTDS, Chennai India for INSA-JRD TATA fellowship during the tenure of this work. YK thanks CSIR for a Senior Research Fellowship. The authors thank Dr. Xavier Foissac (UMR Génomique Diversité et Pouvoir Pathogène, INRAUniversité Victor Ségalen Bordeaux 2, BP81, 33883 Villenave d’Ornon cedex, France and Dr. Matthew Dickinson (School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK) for his useful support. This is IHBT publication number 2071.
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Ayman, O.F., Kumar, Y., Hallan, V. et al. Molecular characterization of the phytoplasmas associated with toon trees and periwinkle in India. J Gen Plant Pathol 76, 351–354 (2010). https://doi.org/10.1007/s10327-010-0257-y
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DOI: https://doi.org/10.1007/s10327-010-0257-y