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Production of hydrophilic phytotoxins by Mycosphaerella fijiensis

  • Fungal Diseases
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Abstract

We have established a reproducible strategy to purify hydrophilic phytotoxins present in the aqueous filtrate of Mycosphaerella fijiensis Morelet. The lyophilized culture filtrate is initially treated with activated charcoal, then successively purified using vacuum liquid chromatography and semipreparative high performance liquid chromatography. Phytotoxic activity was tested using a leaf-spot assay on healthy banana leaves.

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Acknowledgments

This investigation was supported by Project 2004-168 SAGARPA-CONACyT. The authors thank Cecilia Mónica Rodríguez-García and Leticia Peraza-Echeverría (UBT-CICY) for providing the certified strain of M. fijiensis (C-1233), Alberto Mayo-Mosqueda for establishing the in vitro cultures of banana plants, and Silvia Beatriz Andrade-Canto for technical assistance.

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Authors and Affiliations

  1. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, Mérida, 97200, Yucatán, Mexico

    Carlos A. Cruz-Cruz, Karlina García-Sosa, Fabiola Escalante-Erosa & Luis M. Peña-Rodríguez

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  1. Carlos A. Cruz-Cruz
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  2. Karlina García-Sosa
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  3. Fabiola Escalante-Erosa
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  4. Luis M. Peña-Rodríguez
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Correspondence to Luis M. Peña-Rodríguez.

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Cruz-Cruz, C.A., García-Sosa, K., Escalante-Erosa, F. et al. Production of hydrophilic phytotoxins by Mycosphaerella fijiensis . J Gen Plant Pathol 75, 191–195 (2009). https://doi.org/10.1007/s10327-009-0165-1

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  • DOI: https://doi.org/10.1007/s10327-009-0165-1

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