Skip to main content
SpringerLink
Log in

Genetic diversity of Fusarium oxysporum f. sp. spinaciae in Japan based on phylogenetic analyses of rDNA-IGS and MAT1 sequences

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Twenty-eight isolates of Fusarium oxysporum f. sp. spinaciae (FOS; the causal agent of spinach wilt) collected from Japan were assessed for mating type and subjected to phylogenetic analysis. Mating type analysis revealed all isolates to be MAT1-2, suggesting that there is no sexual recombination within the population. Phylogenetic analyses based on nucleotide sequences of the ribosomal DNA intergenic spacer (IGS) and the mating type locus (MAT1) suggested that FOS is polyphyletic. The cluster analysis based on IGS showed four phylogenetic groups (S1–S4) among the isolates. Two distinct lineages, S1 and S3, included FOS isolates both of the vegetative compatibility group (VCG) types, 0330 and 0331, demonstrating that VCG differentiation in FOS may not necessarily reflect the phylogenetic relationships based on IGS and MAT1-2-1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Appel DJ, Gordon TR (1996) Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA. Mol Plant Microbe Interact 9:125–138

    PubMed  CAS  Google Scholar 

  • Arie T, Christiansen SK, Yoder OC, Turgeon BG (1997) Efficient cloning of ascomycete mating type genes by PCR amplification of the conserved MAT HMG box. Fungal Genet Biol 21:118–130

    Article  CAS  PubMed  Google Scholar 

  • Arie T, Yoshida T, Shimizu T, Kawabe M, Yoneyama K, Yamaguchi I (1999) Assessment of Gibberella fujikuroi mating type by PCR. Mycoscience 40:311–314

    Article  CAS  Google Scholar 

  • Arie T, Kaneko I, Yoshida T, Noguchi M, Nomura Y, Yamaguchi I (2000) Mating-type genes from asexual phytopathogenic ascomycetes Fusarium oxysporum and Alternaria alternata. Mol Plant Microbe Interact 13:1330–1339

    Article  PubMed  CAS  Google Scholar 

  • Armstrong GM, Armstrong JK (1976) Common hosts for Fusarium oxysporum formae speciales spinaciae and betae. Phytopathology 66:542–545

    Article  Google Scholar 

  • Barve MP, Arie T, Salimath SS, Muehlbauer FJ, Peever TL (2003) Cloning and characterization of mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp. Fungal Genet Biol 39:151–167

    Article  PubMed  CAS  Google Scholar 

  • Bentley S, Pegg KG, Moore NY, Davis RD, Buddenhagen IW (1998) Genetic variation among vegetative compatibility groups of Fusarium oxysporum f. sp. cubense analyzed by DNA fingerprinting. Phytopathology 88:1283–1293

    Article  CAS  PubMed  Google Scholar 

  • Booth C (1971) The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey

    Google Scholar 

  • Elias KS, Zamir D, Lichtman-Pleban T, Katan T (1993) Population structure of Fusarium oxysporum f. sp. lycopersici: restriction fragment length polymorphisms provide genetic evidence that vegetative compatibility group is an indicator of evolutionary origin. Mol Plant Microbe Interact 6:565–572

    CAS  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (2004) PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle

    Google Scholar 

  • Fernandez D, Assigbetse K, Dubois MP, Geiger JP (1994) Molecular characterization of races and vegetative compatibility groups in Fusarium oxysporum f. sp. vasinfectum. Appl Environ Microbiol 60:4039–4046

    PubMed  CAS  Google Scholar 

  • Fiely MB, Correll JC, Morelock TE (1995) Vegetative compatibility, pathogenicity, and virulence diversity of Fusarium oxysporum recovered from spinach. Plant Dis 79:990–993

    Article  Google Scholar 

  • Fitch WM (1977) On the problem of discovering the most parsimonious tree. Am Nat 111:223–257

    Article  Google Scholar 

  • Fujinaga M, Ogiso H, Shinohara H, Tsushima S, Nishimura N, Togawa M, Saito H, Nozue M (2005) Phylogenetic relationships between the lettuce root rot pathogen Fusarium oxysporum f. sp. lactucae race 1, 2, and 3 based on the sequence of the intergenic spacer region of its ribosomal DNA. J Gen Plant Pathol 71:402–407

    Article  CAS  Google Scholar 

  • Katan T (1999) Current status of vegetative compatibility groups in Fusarium oxysporum. Phytoparasitica 27:51–64

    Google Scholar 

  • Katan T, Di Primo P (1999) Current status of vegetative compatibility groups in Fusarium oxysporum: Supplement (1999). Phytoparasitica 27:273–277

    Google Scholar 

  • Katsube K (1999) Geographic distribution and structure of a field population of Japanese isolates of Fusarium oxysporum f. sp. spinaciae based on vegetative compatibility in Japan. Ann Phytopathol Soc Jpn 65:563–568 (in Japanese with English abstract)

    Google Scholar 

  • Katsube K (2001) Studies on Fusarium wilt of spinach, caused by Fusarium oxysporum f. sp. spinaciae. Res Bull Iwate Agric Res Cent 2:1–60 (in Japanese)

    Google Scholar 

  • Katsube K, Akasaka Y (1997) Control of Fusarium wilt of spinach by transplanting seedlings pretreated with non-pathogenic Fusarium oxysporum. Ann Phytopathol Soc Jpn 63:389–394 (in Japanese with English abstract)

    Google Scholar 

  • Kawabe M, Kobayashi Y, Okada G, Yamaguchi I, Teraoka T, Arie T (2005) Three evolutionary lineages of tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici, based on sequences of IGS, MAT1, and pg1, are each composed of isolates of a single mating type and a single or closely related vegetative compatibility group. J Gen Plant Pathol 71:263–272

    Article  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kistler HC (1997) Genetic diversity in the plant-pathogenic fungus Fusarium oxysporum. Phytopathology 87:474–479

    Article  PubMed  CAS  Google Scholar 

  • Koenig RL, Ploetz RC, Kistler HC (1997) Fusarium oxysporum f. sp. cubense consists of a small number of divergent and globally distributed clonal lineages. Phytopathology 87:915–923

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245

    Article  PubMed  CAS  Google Scholar 

  • Leslie JF (1993) Fungal vegetative compatibility. Annu Rev Phytopathol 31:127–150

    Article  PubMed  CAS  Google Scholar 

  • Lori G, Edel-Hermann V, Gautheron N, Alabouvette C (2004) Genetic diversity of pathogenic and nonpathogenic populations of Fusarium oxysporum isolated from carnation fields in Argentina. Phytopathology 94:661–668

    Article  CAS  PubMed  Google Scholar 

  • Manicom BQ, Baayen RP (1993) Restriction fragment length polymorphisms in Fusarium oxysporum f. sp. dianthi and other fusaria from Dianthus species. Plant Pathol 42:851–857

    Article  CAS  Google Scholar 

  • Mes JJ, Van Doorn J, Roebroeck EJA, Van Egmond E, Van Aartrijk J, Boonekamp PM (1994) Restriction fragment length polymorphisms, races and vegetative compatibility groups within a worldwide collection of Fusarium oxysporum f. sp. gladioli. Plant Pathol 43:362–370

    Article  Google Scholar 

  • O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 95:2044–2049

    Article  PubMed  CAS  Google Scholar 

  • Pöggeler S (1999) Phylogenetic relationships between mating-type sequences from homothallic and heterothallic ascomycetes. Curr Genet 36:222–231

    Article  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing Phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Shimazu J, Yamauchi N, Hibi T, Satou M, Horiuchi S, Shirakawa T (2005) Development of sequence tagged site markers to identify races of Fusarium oxysporum f. sp. lactucae. J Gen Plant Pathol 71:183–189

    Article  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Turgeon BG (1998) Application of matign type gene technology to problems in fungal biology. Annu Rev Phytopathol 36:115–137

    Article  PubMed  CAS  Google Scholar 

  • Vakalounakis DJ, Fragkiadakis GA (1999) Genetic diversity of Fusarium oxysporum isolates from cucumber: differentiation by pathogenicity, vegetative compatibility, and RAPD fingerprinting. Phytopathology 89:161–168

    Article  CAS  PubMed  Google Scholar 

  • Vakalounakis DJ, Wang Z, Fragkiadakis GA, Skaracis GN, Li DB (2004) Characterization of Fusarium oxysporum isolates obtained from cucumber in China by pathogenicity, VCG, and RAPD. Plant Dis 88:645–649

    Article  CAS  Google Scholar 

  • Woudt LP, Neuvel A, Sikkema A, Van Grinsven MQJM, De Milliano WAJ, Campbell CL, Leslie JF (1995) Genetic variation in Fusarium oxysporum from cyclamen. Phytopathology 85:1348–1355

    Article  Google Scholar 

Download references

Acknowledgments

We are grateful to Dr M. Chilvers and Ms. J.E. Stewart for proofreading and suggestion. This study was partly supported by a Grant-in-aid from Japan Society for the Promotion of Science (JSPS) to TA.

Author information

Author notes

  1. Masato Kawabe

    Present address: Applied Biology for Plant Protection Research Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

  2. Kenichi Tsuchiya

    Present address: Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

Authors and Affiliations

  1. Microbial Genetics and Physiology Unit, Department of Biological Safety Microbiology Group, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Masato Kawabe, Takanobu Yoshida & Kenichi Tsuchiya

  2. Department of Management, Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Kazunori Katsube

  3. Institute of Synbiotic Science and Technology, Tokyo University of Agriculture and Technology, Saiwaicho, Fuchu, Tokyo, 183-8509, Japan

    Tsutomu Arie

Authors
  1. Masato Kawabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kazunori Katsube
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takanobu Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tsutomu Arie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kenichi Tsuchiya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masato Kawabe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawabe, M., Katsube, K., Yoshida, T. et al. Genetic diversity of Fusarium oxysporum f. sp. spinaciae in Japan based on phylogenetic analyses of rDNA-IGS and MAT1 sequences. J Gen Plant Pathol 73, 353–359 (2007). https://doi.org/10.1007/s10327-007-0036-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-007-0036-6

Keywords

Search

Navigation