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Elimination of Plum pox virus through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou

  • Viral and Viroid Diseases
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Abstract

The effectiveness of in vitro thermotherapy (explants at 35–37°C for 20 days) in obtaining propagating material of apricot (Prunus armeniaca L.) cultivar Bebecou that is free of Plum pox virus (PPV) was compared to a conventional heat treatment technique (potted plants at 30–35°C for 8 weeks). An improved protocol for in vitro thermotherapy was combined with shoot tip culture. The protocol is 5.8 times more effective than conventional thermotherapy while taking half the time and is likely to have a high commercial impact for nurseries.

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Acknowledgments

The authors would like to thank Dr. Nikolaos Tzortzakis as well as the reviewers for thoughtful recommendations for the improvement of the manuscript.

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Author notes

  1. Georgios C. Koubouris

    Present address: Laboratory of Genetic Improvement, Physiology and Olive Cultivation Techniques, Institute of Olive Tree and Subtropical Plants of Chania, NAGREF, Agrokipio, 73100, Chania, Greece

Authors and Affiliations

  1. Pomology Lab, School of Agriculture, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece

    Georgios C. Koubouris & Miltiadis D. Vasilakakis

  2. Plant Pathology Lab, School of Agriculture, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece

    Varvara I. Maliogka, Kostadinos Efthimiou & Nikolaos I. Katis

Authors
  1. Georgios C. Koubouris
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  2. Varvara I. Maliogka
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  3. Kostadinos Efthimiou
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  4. Nikolaos I. Katis
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  5. Miltiadis D. Vasilakakis
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Correspondence to Georgios C. Koubouris.

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Koubouris, G.C., Maliogka, V.I., Efthimiou, K. et al. Elimination of Plum pox virus through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou. J Gen Plant Pathol 73, 370–373 (2007). https://doi.org/10.1007/s10327-007-0028-6

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  • DOI: https://doi.org/10.1007/s10327-007-0028-6

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