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Involvement of mitogen-activated protein kinase in the induction of StrbohC and StrbohD genes in response to pathogen signals in potato

  • Fungal Diseases
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Abstract

The respiratory burst oxidase homolog (rboh), a plant homolog of the catalytic subunit of phagocyte NADPH oxidase (gp91phox), has been implicated in O2 − generation. In the previous study, we reported that StrbohA is constitutively expressed at a low level, whereas StrbohB is upregulated after pathogen signals are perceived in potato tubers. To gain a better understanding about the role of rboh in the defense response in potato leaves, we investigated the regulatory mechanisms of the gene. StrbohB gene was activated in response to an avirulent race of Phytophthora infestans or to hyphal wall components (HWC) from the pathogen; however, levels of expression were very low. We isolated two novel rboh cDNAs, StrbohC and StrbohD, by virtue of the sequence information from the tomato whitefly-induced gene 1 (LeWfi1) and tomato TC121349, respectively. The expression of StrbohC and StrbohD was drastically upregulated in the potato leaves inoculated with P. infestans or treated with HWC elicitor. Mitogen-activated protein kinase (MAPK) is known to play a pivotal role in the oxidative burst. The transcript accumulation induced by HWC treatment was blocked by U0126, which is a specific inhibitor of MAPK kinase (MAPKK). Conversely, the StrbohC and StrbohD genes were upregulated, driven by a pathogen-inducible promoter, in transgenic potato leaves that carry a constitutively active mutant of the MAPKK, StMEK1DD, that activates defense-related MAPKs. These results suggest that a MAPK cascade is involved in the process leading to the expression of the StrbohC and StrbohD genes.

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Acknowledgments

We deeply thank Takashi Tsuge of Nagoya University for valuable suggestions; Philip M. Mullineaux and Roger P. Hellens of John Innes Centre for pGreen binary vectors; and Miki Yoshioka, Minako Hirano and Ayako Adachi of Nagoya University for cultivation of plants. The authors also thank the members of the Radioisotope Research Center, Nagoya University for technical assistance. This work was supported in part by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists, by a Grant-in-Aid for Scientific Research (S) (14104004) from the Ministry of Education, Science and Culture of Japan and The 21st Century COE Program from the Ministry of Education, Science, Sports and Culture of Japan.

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  1. Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

    Chihiro Yamamizo, Noriyuki Doke, Hirofumi Yoshioka & Kazuhito Kawakita

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  1. Chihiro Yamamizo
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  2. Noriyuki Doke
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  3. Hirofumi Yoshioka
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  4. Kazuhito Kawakita
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Corresponding author

Correspondence to Kazuhito Kawakita.

Additional information

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers AB198716 (StrbohC) and AB198717 (StrbohD).

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Yamamizo, C., Doke, N., Yoshioka, H. et al. Involvement of mitogen-activated protein kinase in the induction of StrbohC and StrbohD genes in response to pathogen signals in potato. J Gen Plant Pathol 73, 304–313 (2007). https://doi.org/10.1007/s10327-007-0020-1

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