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Production of medium chain length polyhydroxyalkanoate from acetate by engineered Pseudomonas putida KT2440

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Pseudomonas putida was metabolically engineered to produce medium chain length polyhydroxyalkanoate (mcl-PHA) from acetate, a promising carbon source to achieve cost-effective microbial processes. As acetate is known to be harmful to cell growth, P. putida KT2440 was screened from three Pseudomonas strains (P. putida KT2440, P. putida NBRC14164, and P. aeruginosa PH1) as the host with the highest tolerance to 10 g/L of acetate in the medium. Subsequently, P. putida KT2440 was engineered by amplifying the acetate assimilation pathway, including overexpression of the acs (encoding acetyl-CoA synthetase) route and construction of the ackA-pta (encoding acetate kinase-phosphotransacetylase) pathway. The acs overexpressing P. putida KT2440 showed a remarkable increase of mcl-PHA titer (+ 92%), mcl-PHA yield (+ 50%), and cellular mcl-PHA content (+ 43%) compared with the wild-type P. putida KT2440, which indicated that acetate could be a potential substrate for biochemical production of mcl-PHA by engineered P. putida.

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Acknowledgements

The authors are very grateful for the kind donation of E. coli MG1655 from Dr. Tao Chen and the plasmid pBBR1MCS-2 from Dr. Yingjin Yuan of Tianjin University (China). The authors wish to acknowledge the financial support provided by the National Basic Research Program of China (Grant No. 2014CB745100), the National Natural Science Foundation of China (Grant No. 21576197), Tianjin Research Program of Application Foundation and Advanced Technology (Grant No. 18JCYBJC23500), and Tianjin Key Research & Development Program (Grant No. 16YFXTSF00460). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Songyuan Yang, Suhang Li & Xiaoqiang Jia

  2. Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, People’s Republic of China

    Songyuan Yang, Suhang Li & Xiaoqiang Jia

  3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People’s Republic of China

    Xiaoqiang Jia

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  1. Songyuan Yang
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Yang, S., Li, S. & Jia, X. Production of medium chain length polyhydroxyalkanoate from acetate by engineered Pseudomonas putida KT2440. J Ind Microbiol Biotechnol 46, 793–800 (2019). https://doi.org/10.1007/s10295-019-02159-5

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  • DOI: https://doi.org/10.1007/s10295-019-02159-5

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