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Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources

  • Synthetic Microbiology and Biotechnology
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Abstract

Production of medium chain length polyhydroxyalkanoate (mcl-PHA) was attempted using Pseudomonas gessardii NIBRBAC000509957, which was isolated from Sunchang, Jeollabuk-do, Republic of Korea (35°24′27.7"N, 127°09′13.0"E) and effectively utilized acetate and formate as carbon sources. We first evaluated the utilization of acetate as a carbon source, revealing optimal growth at 5 g/L acetate. Then, formate was supplied to the acetate minimal medium as a carbon source to enhance cell growth. After overexpressing the acetate and formate assimilation pathway enzymes, this strain grew at a significantly higher rate in the medium. As this strain naturally produces PHA, it was further engineered metabolically to enhance mcl-PHA production. The engineered strain produced 0.40 g/L of mcl-PHA with a biomass content of 30.43% in fed-batch fermentation. Overall, this strain can be further developed to convert acetate and formate into valuable products.

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Acknowledgements

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202327103).

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea

    Woo Young Kim, Seung-Jin Kim & Min-Kyu Oh

  2. Department of Biological Science, Wonkwang University, Iksan, 54538, Republic of Korea

    Hye-rin Seo & Jong-Geol Kim

  3. Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

    Yoonyong Yang, Jong Seok Lee, Moonsuk Hur & Byoung-Hee Lee

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  1. Woo Young Kim
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Contributions

Woo Young Kim: Conceptualization, methodology, validation, formal analysis, investigation, data curation, writing the original draft, and visualization. Hye-rin Seo, Jong-Geol Kim: Conceptualization, methodology, and writing. Seung-Jin Kim, Yoonyong Yang, Jong Seok Lee, Moonsuk Hur, Byoung-Hee Lee: Conceptualization, methodology, writing, and reviewing. Min-Kyu Oh: Conceptualization, methodology, validation, writing–review and editing, supervision, project administration, and funding acquisition.

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Correspondence to Min-Kyu Oh.

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Kim, W.Y., Kim, SJ., Seo, Hr. et al. Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources. J Microbiol. (2024). https://doi.org/10.1007/s12275-024-00136-x

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