Abstract
Utilization of renewable feedstocks for the production of bio-based bulk chemicals, such as 2,3-butanediol (2,3-BDO), by engineered strains of the non-pathogenic yeast, Saccharomyces cerevisiae, has recently become an attractive option. In this study, to realize rapid production of 2,3-BDO, a flocculent, 2,3-BDO-producing S. cerevisiae strain YPH499/dPdAdG/BDN6-10/FLO1 was constructed from a previously developed 2,3-BDO-producing strain. Continuous 2,3-BDO fermentation was carried out by the flocculent strain in an airlift bioreactor. The strain consumed more than 90 g/L of glucose, which corresponded to 90% of the input, and stably produced more than 30 g/L of 2,3-BDO over 380 h. The maximum 2,3-BDO productivity was 7.64 g/L/h at a dilution rate of 0.200/h, which was higher than the values achieved by continuous fermentation using pathogenic bacteria in the previous reports. These results demonstrate that continuous 2,3-BDO fermentation with flocculent 2,3-BDO-producing S. cerevisiae is a promising strategy for practical 2,3-BDO production.
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References
Bialkowska AM (2016) Strategies for efficient and economical 2,3-butanediol production: new trends in this field. World J Microbiol Biotechnol 32:200
Bony M, Thines-Sempoux D, Barre P, Blondin B (1997) Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p. J Bacteriol 179:4929–4936
Caro LH, Tettelin H, Vossen JH, Ram AF, van den Ende H, Klis FM (1997) In silicio identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae. Yeast 13:1477–1489
Celinska E, Grajek W (2009) Biotechnological production of 2,3-butanediol: current state and prospects. Biotechnol Adv 27:715–725
Chen DC, Yang BC, Kuo TT (1992) One-step transformation of yeast in stationary phase. Curr Genet 21:83–84
Couto SR, Toca-Herrera JL (2007) Laccase production at reactor scale by filamentous fungi. Biotechnol Adv 25:558–569
Ji XJ, Huang H, Ouyang PK (2011) Microbial 2,3-butanediol production: a state-of-the-art review. Biotechnol Adv 29:351–364
Jullesson D, David F, Pfleger B, Nielsen J (2015) Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals. Biotechnol Adv 33:1395–1402
Kim JW, Kim J, Seo SO, Kim KH, Jin YS, Seo JH (2016) Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities. Biotechnol Biofuels 9:265
Kim S, Hahn JS (2015) Efficient production of 2,3-butanediol in Saccharomyces cerevisiae by eliminating ethanol and glycerol production and redox rebalancing. Metab Eng 31:94–101
Kim SJ, Seo SO, Jin YS, Seo JH (2013) Production of 2,3-butanediol by engineered Saccharomyces cerevisiae. Bioresour Technol 146:274–281
Lee HK, Maddox IS (1986) Continuous production of 2,3-butanediol from whey permeate using Klebsiella pneumoniae immobilized in calcium alginate. Enzyme Microb Technol 8:409–411
Ng CY, Jung MY, Lee J, Oh MK (2012) Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering. Microb Cell Fact 11:68
Nordström K (1968) Yeast growth and glycerol formation II. Carbon and redox balances. J Inst Brew 74:429–432
Ostergaard S, Olsson L, Nielsen J (2000) Metabolic engineering of Saccharomyces cerevisiae. Microbiol Mol Biol Rev 64:34–50
Ramachandran KB, Goma G (1987) Effect of oxygen supply and dilution rate on the production of 2,3-butanediol in continuous bioreactor by Klebsiella pneumoniae. Enzyme Microb Technol 9:107–111
Sikorski RS, Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19–27
Smukalla S, Caldara M, Pochet N, Beauvais A, Guadagnini S, Yan C, Vinces MD, Jansen A, Prevost MC, Latge JP, Fink GR, Foster KR, Verstrepen KJ (2008) FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast. Cell 135:726–737
Van Mulders SE, Christianen E, Saerens SM, Daenen L, Verbelen PJ, Willaert R, Verstrepen KJ, Delvaux FR (2009) Phenotypic diversity of Flo protein family-mediated adhesion in Saccharomyces cerevisiae. FEMS Yeast Res 9:178–190
Verbelen PJ, De Schutter DP, Delvaux F, Verstrepen KJ, Delvaux FR (2006) Immobilized yeast cell systems for continuous fermentation applications. Biotechnol Lett 28:1515–1525
Westman JO, Mapelli V, Taherzadeh MJ, Franzen CJ (2014) Flocculation causes inhibitor tolerance in Saccharomyces cerevisiae for second-generation bioethanol production. Appl Environ Microbiol 80:6908–6918
Wong CL, Yen HW, Lin CL, Chang JS (2014) Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain. Bioresour Technol 152:169–176
Xie D, Shao Z, Achkar J, Zha W, Frost JW, Zhao H (2006) Microbial synthesis of triacetic acid lactone. Biotechnol Bioeng 93:727–736
Xue C, Zhao X-Q, Liu C-G, Chen L-J, Bai F-W (2013) Prospective and development of butanol as an advanced biofuel. Biotechnol Adv 31:1575–1584
Yamada R, Taniguchi N, Tanaka T, Ogino C, Fukuda H, Kondo A (2010) Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. Microb Cell Fact 9:32
Yamada R, Wakita K, Mitsui R, Nishikawa R, Ogino H (2017) Efficient production of 2,3-butanediol by recombinant Saccharomyces cerevisiae through modulation of gene expression by cocktail delta-integration. Bioresour Technol 245:1558–1566
Zeng A-P, Biebl H, Deckwer W-D (1990) 2,3-Butanediol production by Enterobacter aerogenes in continuous culture: role of oxygen supply. Appl Microbiol Biotechnol 33:264–268
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A part of this work was supported by a New Chemical Technology Research Encouragement Award from the Japan Association for Chemical Innovation and by a Grant for Basic Science Research Projects from the Sumitomo Foundation.
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Yamada, R., Nishikawa, R., Wakita, K. et al. Rapid and stable production of 2,3-butanediol by an engineered Saccharomyces cerevisiae strain in a continuous airlift bioreactor. J Ind Microbiol Biotechnol 45, 305–311 (2018). https://doi.org/10.1007/s10295-018-2033-5
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DOI: https://doi.org/10.1007/s10295-018-2033-5