Improved cider fermentation performance and quality with newly generated Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids
Yeast cryotolerance may be advantageous for cider making, where low temperatures are usually employed. Here, we crossed the cryotolerant S. eubayanus with a S. cerevisiae wine strain and assessed the suitability of the hybrids for low-temperature cider fermentation. All strains fermented the juice to 5% ABV, but at different rates; hybrid strains outperformed S. cerevisiae, which was sensitive to low temperatures. The best hybrid fermented similarly to S. eubayanus. S. eubayanus produced sulphurous off flavours which masked a high concentration of fruity ester notes. This phenotype was absent in the hybrid strains, resulting in distinctly fruitier ciders. Aroma was assessed by an independent consumer panel, which rated the hybrid ciders as identical to the wine strain cider. Both were significantly more pleasant than the S. eubayanus cider. Interspecific hybridization can apparently be used effectively to improve low-temperature fermentation performance without compromising product quality.
KeywordsSaccharomyces eubayanus Cider Hybridization Cryotolerance
The temperature employed in alcoholic beverages fermentations impacts the sensorial properties of the final product. There are several reasons why cold fermentations (i.e., lower than 15 °C) are preferred over warm fermentations. In brewing, these conditions allow for the production of the clean and fresh tasting lager beer, which has contributed to the global popularity of this beer style . Contrarily, in white wine making, low temperature actually improves the aromatic profile of the wine [9, 10, 40]. Compounds like ethyl esters associated with fruit notes and freshness tend to be produced and maintained at higher concentrations in white wines fermented at low temperatures . The reduced evaporation of volatile compounds in the cold must also be taken into consideration. Other than the sensorial properties, the use of low temperatures also reduces the likelihood of contamination by spoilage organisms, potentially obviating the use of SO2. It is, however, necessary to have a yeast strain that is able to survive and retain metabolic activity at these temperatures if fermentation is to proceed efficiently.
Naturally cold-tolerant strains like Saccharomyces kudriavzevii, S. uvarum or S. eubayanus could potentially be used for low-temperature fermentations, but tend to have higher ethanol sensitivity than S. cerevisiae and may, therefore, be less suitable for alcoholic fermentation . It is not uncommon, however, to find hybrids of S. cerevisiae and a cold-tolerant Saccharomyces species associated with alcoholic fermentations. From the best-known case of the lager yeast S. cerevisiae × S. eubayanus hybrids [15, 26], to hybrids involving S. cerevisiae, S. uvarum and/or S. kudriavzevii in cider fermentations [18, 35, 42].
Since the discovery of S. eubayanus in Patagonia  there has been an interest in recreating the hybridization event and generating new lager strains for brewing applications [22, 24, 25, 37]. The discovery has also inspired new approaches to facilitate the selection of artificial hybrid strains . Lager yeast hybrids have inherited the superior fermentation performance from their S. cerevisiae parent and the cold tolerance from S. eubayanus. This has resulted in strains that have superior phenotypic properties relative to their parents, a phenomenon called heterosis .
Cold tolerance seems the main phenotypic contribution of S. eubayanus to the natural and artificial lager yeast strains [22, 25, 37], but the presence of an additional high-affinity fructose transporter, Fsy1, could provide hybrids with improved ability to reduce the critical accumulation of fructose in the later phases of the wine and cider fermentations . Fructose represents the larger portion of fermentable sugars in apple juice, being usually present in concentration two times higher than glucose .
Countries like France, Spain, Ireland and Slovenia have a strong cider culture and this is traditionally produced with naturally occurring indigenous yeast at temperatures ranging from 12 to 20 °C . In the initial stages of cider production species from the genus Candida, Pichia, Hansenula, Hanseniaspora and Metschnikowia predominate, having a strong influence in the aromatic complexity of the cider [12, 39, 41, 55]. The alcoholic fermentation, however, is carried by Saccharomyces species .
There has been an increased interest in cider production recently, particularly in North America but also in Eastern European countries without a tradition for cider consumption. In 2015 alone, cider production, for example, grew by 106% in Poland. This accelerated production was most likely a result of a search for an alternative use for apples after the Russian ban on apple importing from Poland combined with record production levels and decrease in excise duties . The increased popularity of cider can also be attributed to consumer tastes changing towards less-bitter drinks, with the addition of being gluten-free and lower in alcohol than wine.
In this work, we describe the generation of new interspecific hybrids between an S. cerevisiae wine strain and S. eubayanus type strain and characterised the potential of these hybrids for cider fermentations. The ciders were analysed for their aromatic properties and were also evaluated by a consumer panel.
Materials and methods
List of strains used in this study
Haploid derivative of industrial wine strain Lalvin EC1118 
VTT C-12902 (C902)
S. eubayanus type strain; CBS12357 
VTT C-16962 (C962)
59A × C902 hybrid
VTT C-16963 (C963)
59A × C902 hybrid
VTT C-16964 (C964)
59A × C902 hybrid
VTT C-16965 (C965)
59A × C902 hybrid
VTT C-16966 (C966)
59A × C902 hybrid
VTT C-16967 (C967)
59A × C902 hybrid
Prior to hybridization, natural lysine auxotrophic mutants of S. cerevisiae 59A were selected based on their ability to grow on α-aminoadipic acid-containing agar . Lack of growth in minimal selection medium confirmed the auxotrophy .
Hybrids between S. cerevisiae 59A lys- and S. eubayanus C902 were generated by rare mating as described in Ref. . A fresh colony of each parent was used to inoculate YPD cultures that were grown overnight at 25 °C. The cultures were centrifuged at 5000×g for 5 min and the cells were washed and re-suspended in sterile H2O to a concentration of 10 g L−1. In a sterile 2 mL Eppendorf tube 100 µL of each suspension were added followed by 1 mL of YPD medium. The procedure was performed in triplicate. The tubes were vortexed and incubated statically for 5 days at 25 °C. Finally, the suspension was washed and re-suspended in 500 µL of starvation medium (0.1% yeast extract and 0.1% glucose) and incubated for 2 h at room temperature.
For selection of prototrophic hybrid cells, the suspension was vortexed and 100 µL were added per minimal selection agar (without lysine) plate. These plates were incubated at 37 °C (an inhibitory temperature for S. eubayanus) and the hybrids were selected based on the restoration of prototrophy and ability to grow at 37 °C.
Confirmation of hybrid status
Colonies were checked for their hybrid status by rDNA-PCR amplification using the primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′), followed by a digestion using HaeIII (New England Biolabs, Ipswich, MA, USA) . Hybrids can be identified by having the ITS regions of both parents and thus the restriction profile appears as a combination of the parental profiles. Briefly, S. cerevisiae yields a 4 band pattern (320, 225, 180, 140 bp), S. eubayanus 3 bands (490, 225, 140 bp) and interspecific hybrids 5 bands (490, 320, 225, 180 and 140 bp). In addition, strain specific primers were used to confirm the presence of DNA from both parents, MEX67 for S. cerevisiae 59A, ScerF2 (5′-GCGCTTTACATTCAGATCCCGAG-3′) and ScerR2 (5′-TAAGTTGGTTGTCAGCAAGATTG-3′), and a putative maltose transporter for S. eubayanus C902, SeubF4 (5′-CGATGAAGGGCTTATCCTCACTG-3′) and SeubF5 (5′-CGAGATGGTGTGCTTCGCC-3′).
Ploidy determination by FACS
The DNA content was determined by flow cytometry essentially as described by Haase and Reed . The parental strain S. cerevisiae 59A forms aggregates that impair an accurate measurement of the ploidy and so a strains deleted on AMN1 gene was used . Cells were grown overnight in YPD medium (1% yeast extract, 2% peptone, 2% glucose), and approximately 1 × 107 cells were washed with 1 mL of 50 mM citrate buffer. Cells were fixed with cold 70% ethanol and incubated at room temperature for 1 h. Cells were then washed with 50 mM citrate buffer, resuspended in 50 mM citrate buffer containing 0.25 mg mL−1 RNAse A and incubated overnight at 37 °C. 1 mg mL−1 of Proteinase K was then added, and cells were incubated for 1 h at 50 °C. Cells were then stained with SYTOX Green (2 μM; Life Technologies, USA), and their DNA content was determined using a FACSAria cytometer (Becton–Dickinson). DNA contents were calculated by comparing fluorescence intensities with those of S. cerevisiae haploid (CEN.PK113-1A) and diploid (CEN.PK) reference strains. Measurements were performed on duplicate independent yeast cultures, and 100,000 events were collected per sample during flow cytometry.
Origin of mitochondria
The origin of the mitochondria inherited by the hybrids was assessed by sequencing of the COX2 gene. The gene was amplified by PCR using the following primers: COX2 (forward): GGTATTTTAGAATTACATGA and COX2 (reverse): ATTTATTGTTCRTTTAATCA as described in Ref. . PCR products were purified with QIAquick PCR purification kit (Qiagen) and sequenced by GATC-Biotech using the same primers. Gene sequences were aligned using MUltiple Sequence Comparison by Log-Expectation (MUSCLE; http://www.ebi.ac.uk/Tools/msa/muscle/). The functionality of mitochondria was assessed by plating a fresh colony onto a YP plate containing 3% (w/v) glycerol as the sole carbon source.
The range of growing temperatures was determined by drop test. A culture of each strain was grown overnight in YPD medium. The OD600nm of each culture was adjusted to 0.5 and three consecutive tenfold dilutions were made (0.05, 0.005 and 0.0005). 5 µL of each dilution were spotted in several plates and these were incubated at temperatures of 7, 10, 20, 28 and 37 °C and allowed to grow for 2 days at 28 and 37 °C, 3 days at 20 °C, 5 days at 12 °C and 7 days at 4 °C.
Fructose and glucose uptake
For fructose and glucose uptake measurement, the yeast strains were grown at 20 °C in liquid YP medium containing glucose (2% w/v) to an OD600nm between 4 and 8. The cells were harvested by centrifugation (10 min, 5000 rpm, 0 °C), washed with ice-cold water and 0.1 M tartrate-Tris (pH 4.2) and re-suspended in the same buffer to a concentration of 200 mg fresh yeast mL−1. Zero-trans rates of [U-14C]-fructose and [U-14C]-glucose uptake at 20 °C were determined with 5 mM of substrate with 10 s incubation time . Two incubation times were tested to ensure linearity with respect to time with t2 corresponding to at least 90% of t1 value. [U-14C]-Fructose (ART0329) was from American Radiolabelled Chemicals (St. Louis, MO, USA) and [U-14C]-glucose (CFB 96) was from Amersham Biosciences (Buckinghamshire, UK).
Apple juice fermentation
Apple juice fermentation was carried out with a similar setup as previously described for brewer’s wort [16, 47]. A concentrated mixture of apple and pear juice (74%; Finlandia cider, Senson Oy, Lahti, Finland) was used as substrate for cider fermentations. The concentrate contains sulphur dioxide, ammonium phosphate and the sweetener acesulphame K. Juice was diluted to a concentration of approximately 11 °Brix (64 g L−1 fructose and 32 g L−1 glucose). 1.5 L of 11 °Brix juice was fermented with selected strains in stainless steel cylindroconical vessels (so-called tall tubes) with dimensions of 6 cm internal diameter × 100 cm height (two litres) at a pitching rate of 3 g of fresh yeast L−1. Starting cultures were prepared by inoculating a fresh colony in 50 mL YPD and grown for 24 h at 25 °C, 150 rpm. The culture was then diluted in 500 mL 11 °Brix apple juice to an initial OD of 0.1 and grown for 2 days. The fermentations were carried out at 10 and 20 °C until no change in the residual extract was observed for 24 h.
Samples were withdrawn daily, centrifuged at 9000 rpm for 10 min at 1 °C and the pellet was washed with water, weighed, re-suspended in water and used for yeast analyses. Dry yeast masses were determined by drying the washed yeast slurry overnight at 105 °C in a pre-weighed porcelain crucible. Specific gravity, alcohol level and pH were determined from the centrifuged and degassed fermentation samples using an Anton Paar density meter DMA 5000 M with Alcolyzer Beer ME and pH ME modules (Anton Paar GmbH, Austria).
Concentration of fermentable sugars (glucose and fructose) was measured by HPLC using a waters 2695 separation module and waters system interface module liquid chromatograph coupled with a waters 2414 differential refractometer (Waters Co., Milford, MA, USA). An Aminex HPX-87H organic acid analysis column (300 × 7.8 mm, Bio-Rad) was equilibrated with 5 mM H2SO4 (Titrisol, Merck, Germany) in water at 55 °C and samples were eluted with 5 mM H2SO4 in water at a 0.3 ml min−1 flow rate.
Quantification of volatile compounds
Fourteen yeast-derived volatile compounds were determined by headspace gas chromatography with flame ionisation detector (HSGC-FID) analysis. Four-millilitre samples were filtered (0.45 μm) and incubated at 60 °C for 30 min, and then 1 mL of gas phase was injected (split mode; 225 °C; split flow of 30 mL min−1) into a gas chromatograph equipped with an FID detector and headspace autosampler (Agilent 7890 Series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50 m × 320 μm × 1.05 μm column; Agilent, USA). The carrier gas was helium (constant flow of 1.4 mL min−1). The temperature programme was 50 °C for 3 min, 10 °C min−1–100 °C, 5 °C min−1–140 °C, 15 °C min−1–260 °C and then isothermal for 1 min. Compounds were identified by comparison with authentic standards and were quantified using standard curves. 1-Butanol was used as internal standard.
Twelve sulphur volatile compounds (SVC) in the ciders produced at 20 °C were quantified by Campden BRI (Nutfield, Surrey, UK) by purge-and-trap linked to GC with chemiluminescence detection. The sample was purged with helium into a Tekmar–Dohrmann sample concentrator then the trapped volatiles were desorbed and injected into the GC coupled with a sulphur-specific Sievers chemiluminescence detector. Sulphur volatiles were individually calibrated against reference standards.
To assess the quality of the ciders produced by the hybrids relative to the parents, a panel of volunteer consumers rated the pleasantness of aroma on a scale of 1 (dislike extremely) to 9 (like extremely) of the ciders produced at 20 °C. The panel consisted of 45 women and 13 men between 18 and 65 years old from the southwest of Finland. Data were collected using Compusense Cloud (Compusense Inc. Ontario, Canada). The study was conducted at the sensory laboratory of the Functional Foods Forum (ISO 8589), University of Turku.
Statistical analysis was performed with R (http://www.rproject.org/; v3.3.0) using one-way ANOVA and Tukey test. Visualisation of data in heat-maps was done with the package ggplot based on z scores. The z scores (z) were calculated as z = (x−μ)/σ, where x is the concentration of an aroma compound in a particular cider sample, μ is the mean concentration of that aroma compound in all ciders, and σ is the standard deviation of concentration of that aroma compound in all ciders.
Confirmation of hybrid status, ploidy and mitochondria determination
The colonies growing in selective conditions were regarded as potential hybrids and were screened through ITS PCR followed by digestion with HaeIII and species specific PCR. The 5 band digestion pattern (Fig. S1a) and the 2 band amplification (Fig. S1b) confirmed the presence of genetic material from the two different species. Additionally, the presence of chromosomes from both parents can be seen in the karyotype (Fig. S1c).
The ploidy measurements confirmed that S. cerevisiae 59A is haploid (1.01n) and S. eubayanus diploid (2.07n) (Fig. S2, Table 1). The hybrids were found to be roughly diploid but with small variation in DNA content between them, ranging from 2.07n to 2.38n (Fig. S2, Table 1). Sequencing of COX2 gene revealed that hybrids C962, C963 and C964 contain S. cerevisiae mitochondrial DNA (Table 1; Fig S3). PCR using DNA from the strains C965, C966 and C967 did not yield any product in several attempts, suggesting defective or absent mitochondria. The functionality of the mitochondria was evaluated by testing the ability to grow in medium YP containing glycerol as carbon source. This assay confirmed the inability of hybrids C965–C967 to grow on non-fermentable carbon sources indicating the absence of functional mitochondria (Fig. S4).
Temperature tolerance and uptake of the hexoses glucose and fructose
Growth temperature and sugar uptake are the major factors affecting the fermentation rate. We tested the range of growth temperatures by drop test and the uptake of radiolabelled glucose and fructose.
Fermentation in cider-making conditions
Cider quality assessment
Concentration of sulphur volatile compounds (SVC’s) in µg L−1 in the ciders produced at 20 °C (± standard deviation of two biological replicates)
0.44 ± 0.16
0.35 ± 0.07
0.35 ± 0.35
0.55 ± 0.07
0.65 ± 0.35
0.35 ± 0.07
0.10 ± 0.14
0.15 ± 0.21
2.34 ± 0.74
0.12 ± 0.16
1.15 ± 0.35
0.15 ± 0.07
0.05 ± 0.07
0.30 ± 0.00
0.20 ± 0.00
0.30 ± 0.14
0.20 ± 0.00
1.15 ± 0.50
1.10 ± 0.07
0.65 ± 0.07
0.30 ± 0.14
1.55 ± 0.50
1.50 ± 0.14
0.90 ± 0.14
0.30 ± 0.42
The use of hybrid yeast involving S. eubayanus is still limited to lager beer brewing. The lager yeast are, however, a group with rather low genetic and phenotypic diversity . The limited aroma spectrum of lager strains is desirable for lager beers, where a clean aroma is generally preferred . Ciders, however, benefit from a more complex profile. Wine strains represent a more diverse group of strains and therefore a more interesting alternative for cider making. After crossing a wine strain with S. eubayanus, hybrid strains were expected to inherit the more pleasant aroma profile of the former and the reportedly high tolerance to low temperatures of the latter .
We obtained diploid hybrid strains with variable performance in apple juice, with the best strain performing similarly to S. eubayanus and producing comparable amounts of aroma compounds but with the advantage of producing SVCs at concentrations under the detection level.
Hybrids generated by rare mating often contain the complete DNA of both parents [19, 24]; this was, however, not the case here as the hybrids were diploid rather than triploid. This may be either caused by the high sporulation capacity of S. eubayanus  that resulted in a spore of C902 mating with S. cerevisiae 59A, or by loss of chromosomes post-hybridization due to chromosome instability and even incompatibility. Examples of the latter are the chromosome III from S. cerevisiae that has high loss frequency  and the chromosome XII from S. bayanus being partially incompatible with the S. cerevisiae genome . This phenomenon seems to be strain and species specific [28, 32]. S. cerevisiae × S. uvarum and S. cerevisiae × S. kudriavzevii hybrids show different rates of chromosome loss with hybrids involving S. kudriavzevii being more unstable . The chromosomes from the non-S. cerevisiae parent tend to be have higher loss frequency [43, 44]. However, the ploidy of the S. cerevisiae × S. eubayanus hybrids generated in this study was measured shortly after purification and confirmation in the hybrid state and, thus, such rearrangements of the hybrid genome are unlikely to have had the chance to occur. The alternative, i.e., fusion of a haploid cell and haploid spore may therefore be more plausible. The diploid state may explain why only single parent transgression in fermentation was observed. Recent studies on artificial hybrids with different ploidies have shown a direct and positive relationship between DNA content and fermentation rate .
The phenotypic variations among hybrid strain were visible already in the range of growth temperatures. An ability to grow at high temperatures (37 °C), a property not possessed by S. eubayanus C902, was expected as this was one of the selection factors. However, at the lower range of 4–12 °C, the strain C964 was clearly different to the others. The mechanisms of cold tolerance of S. eubayanus are still unclear . One possible explanation is that the genome of S. eubayanus may be more dominant in this hybrid strain which also shows a similar fermentation profile; however, that is not yet known. A higher proportion of S. eubayanus genetic material in lager yeast hybrids appears to relate with the cold tolerance of these strains .
The non-Mendelian nature of mitochondria inheritance can also influence the phenotypic properties of new hybrid strains. The hybrids described in this study either contain S. cerevisiae mitochondria (C962–C964) or no mitochondria was found (C965–C967). The hybrids containing S. cerevisiae mitochondria showed superior hexose transport, but the advantage to fermentation performance was only observed at low temperature fermentation (10 °C), possibly due to the reduced influence of mitochondria on fermentative behaviour . However, S. cerevisiae × S. uvarum hybrids that retain the S. cerevisiae mitochondrial genome have lower expression of genes related to hexose transport and glycolysis/fermentation pathways . Nevertheless, the same study also suggests that the inheritance of mitochondrial DNA is strain specific and therefore hybrids obtained from repeated crosses with the same parental strains will retain the same mtDNA . This seems to be true for lager strains, where only S. eubayanus type mitochondria were found independently of the strain group [13, 45]. When hybrids are generated artificially, the origin of mitochondrial DNA varies even when using the same parental strains . A more recent study showed that, in addition to the strain background, environmental conditions also influence the origin of the mtDNA retained in interspecific hybrids . This is also, however, unlikely to explain the differences observed between the hybrids generated in this work, since these were maintained in similar conditions post-hybridization and during hybrid isolation. The most likely explanation for the loss of mitochondria may be that these 3 hybrids (C965–C967) initially contained S. eubayanus mitochondria, but during the hybrid isolation process the mitochondria were lost due to the high temperatures employed (37 °C). At this stage, the hybrid genomes are unstable and such temperature conditions may have induced the loss of S. eubayanus mitochondria, considering 37 °C is a restrictive temperature for S. eubayanus. Non-permissive temperatures have been shown to restrict the transmission of mitochondria in S. cerevisiae strains with mutations in MDM1 and MDM2 genes . These results suggest that there is still work to do to understand which factors influence the inheritance and the retention of functional mitochondria in interspecies hybrids.
The sugar uptake kinetics of S. eubayanus C902 are superior to those of S. cerevisiae 59A and, while the hybrids show lower uptake rates than S. eubayanus, these are still generally more efficient than S. cerevisiae in transporting glucose and fructose and this is reflected in the fermentation performance. The mechanism responsible is, however, not clear as little is known about hexose transport kinetics of S. eubayanus. In S. cerevisiae, HXT transporters are induced/repressed at varying concentrations of glucose, i.e., low affinity HXT1 and HXT3 are induced by high glucose levels while high-affinity HXT2 and HXT4-7 are induced by low glucose concentration and are essential at the end of fermentation together with FSY1 for fructose uptake [5, 14, 27, 29]. The latter gene is present in both parent strains and they share 78% similarity at the amino acid level . The activity of Fsy1 symporter may be relevant for fructose scavenging in the later stages of fermentation as it is repressed at high fructose concentrations and induced by non-fermentable carbon sources such as ethanol [5, 14].
A disadvantage of using S. eubayanus for industrial fermentations is that it produces perceptible amounts of unpleasant sulphur volatiles. These completely masked the fruit and floral notes of the esters and higher alcohols, despite these being produced in much higher concentrations than in the S. cerevisiae fermentations. Unpleasant sulphur-like notes in beers produced with S. eubayanus strains have similarly been described before by Mertens and coworkers . These were also apparently absent in hybrids produced with S. cerevisiae strains. Hybridization approaches for the removal of 4-vinylguaiacol (phenolic off-flavour) and H2S have likewise been described [11, 53]. The production of SVCs by S. eubayanus may be due to differential amino acid uptake. We noted that S. eubayanus C902 has greater nitrogen requirements than S. cerevisiae 59A and the hybrids (data not shown). In nitrogen-deficient conditions, the sulphate reduction sequence pathway is triggered to meet the yeast demand in cysteine and methionine, this results in accumulation of sulphide that is then released from the cells as H2S . H2S reacts with ethanol or acetaldehyde to form ethanethiol which can be oxidised to diethyl disulphide [4, 46, 49] while methanethiol is formed by degradation of methionine .
Phenotypically, the hybrids display an interesting combination of the parental properties indicating potential for cider fermentation and possibly further applications. A wider range of temperatures permitting growth could be used to modulate the properties of the final product. It was shown that the aromatic profile is substantially different at fermentation temperatures of 10 and 20 °C in accordance with what is observed in brewing fermentations with natural lager hybrids . Mass production of these yeast strains may also be facilitated as tolerance of temperatures as high as 37 °C is likely to facilitate production in the form of active dried yeast . In addition, the high hexose uptake rates suggest that S. eubayanus and the hybrids could be advantageous for low-temperature fermentation of other sugar-rich substrates such as grape must.
Eero Mattila is thanked for assistance in the VTT Pilot Brewery, and Aila Siltala for skilled technical assistance. Senson Oy is thanked for the donation of the fruit juice used in fermentations. We are greatful for Saila Mattila for conducting consumer test data collection in the FFF sensory laboratory, and also for volunteer consumers participating the study. This work was supported by the Academy of Finland (Academy Project 276480), the European Union’s Seventh Framework Programme FP7/2007-2013/under REA Grant Agreement no. 606795 and Alfred Kordelin Foundation, Svenska Kulturfonden-The Swedish Cultural Foundation in Finland.
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