Skip to main content

Advertisement

Log in

Sweet sorghum bagasse and corn stover serving as substrates for producing sophorolipids

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

To make the process of producing sophorolipids by Candida bombicola truly sustainable, we investigated production of these biosurfactants on biomass hydrolysates. This study revealed: (1) yield of sophorolipds on bagasse hydrolysate decreased from 0.56 to 0.54 and to 0.37 g/g carbon source when yellow grease was dosed at 10, 40 and 60 g/L, respectively. In the same order, concentration of sophorolipids was 35.9, 41.9, and 39.3 g/L; (2) under similar conditions, sophorolipid yield was 0.12, 0.05 and 0.04 g/g carbon source when corn stover hydrolysate was mixed with soybean oil at 10, 20 and 40 g/L. Sophorolipid concentration was 11.6, 4.9, and 3.9 g/L for the three oil doses from low to high; and (3) when corn stover hydrolysate and yellow grease served as the substrates for cultivating the yeast in a fermentor, sophorolipid concentration reached 52.1 g/L. Upon further optimization, sophorolipids production from ligocellulose will be indeed sustainable.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Chen X, Shekiro J, Pschorn T, Sabourin M, Tao L, Elander R, Tucker MP (2014) A highly efficient dilute alkali deacetylation and mechanical (disc) refining process for the conversion of renewable biomass to lower cost sugars. Biotechnol Biofuels 7(1):98

    Article  Google Scholar 

  2. Choudhary R, Umagiliyage AL, Liang Y, Siddaramu T, Haddock J, Markevicius G (2012) Microwave pretreatment for enzymatic saccharification of sweet sorghum bagasse. Biomass Bioenergy 39:218–226

    Article  CAS  Google Scholar 

  3. Ciesielska K, Van Bogaert IN, Chevineau S, Li B, Groeneboer S, Soetaert W, Van de Peer Y, Devreese B (2014) Exoproteome analysis of Starmerella bombicola results in the discovery of an esterase required for lactonization of sophorolipids. J Proteom 98:159–174

    Article  CAS  Google Scholar 

  4. Daniel H-J, Reuss M, Syldatk C (1998) Production of sophorolipids in high concentration from deproteinized whey and rapeseed oil in a two stage fed batch process using Candida bombicola ATCC 22214 and Cryptococcus curvatus ATCC 20509. Biotechnol Lett 20:1153–1156

    Article  CAS  Google Scholar 

  5. Davila A-M, Marchal R, Vandecasteele J-P (1992) Kinetics and balance of a fermentation free from product inhibition: sophorose lipid production by Candida bombicola. Appl Microbiol Biotechnol 38:6–11

    Article  CAS  Google Scholar 

  6. Davila A-M, Marchal R, Vandecasteele J-P (1997) Sophorose lipid fermentation with differentiated substrate supply for growth and production phases. Appl Microbiol Miotechnol 47:496–501

    Article  CAS  Google Scholar 

  7. Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61(1):47–64

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Hillion G, Marchal R, Stoltz C, Borzeix F (1998) Use of a sophorolipid to provide free radical formation inhibiting activity or elastase inhibiting activity. Google Patents, US5756471 A

  9. Hommel R, Weber L, Weiss A, Himmelreich U, Rilke O, Kleber H-P (1994) Production of sophorose lipid by Candida (Torulopsis) apicola grown on glucose. J Biotechnol 33:147–155

    Article  CAS  Google Scholar 

  10. Huang TT (2013) Chemically modified sophorolipids and uses thereof. Google Patents, WO2013003291 A3

  11. Huang TT (2014) Carbohydrate esters as inducers for gene expression. Google Patents, WO2014015179 A1

  12. Isoda H, Kitamoto D, Shinmoto H, Matsumura M, Nakahara T (1997) Microbial extracellular glycolipid induction of differentiation and inhibition of the protein kinase C activity of human promyelocytic leukemia cell line HL60. Biosci Biotechnol Biochem 61:609–614

    Article  CAS  PubMed  Google Scholar 

  13. Klekner V, Kosaric N, Zhou Q (1991) Sophorose lipids produced from sucrose. Biotechnol Lett 13:345–348

    Article  CAS  Google Scholar 

  14. Liang Y, Jarosz K, Wardlow AT, Zhang J, Cui Y (2014) Lipid production by Cryptococcus curvatus on hydrolysates derived from corn fiber and sweet sorghum bagasse following dilute acid pretreatment. Appl Biochem Biotechnol 173:2086–2098

    Article  CAS  PubMed  Google Scholar 

  15. Liang Y, Perez I, Goetzelmann K, Trupia S (2014) Microbial lipid production from pretreated and hydrolyzed corn fiber. Biotechnol Prog 30:945–951

    Article  CAS  PubMed  Google Scholar 

  16. Liang Y, Sarkany N, Cui Y, Yesuf J, Trushenski J, Blackburn JW (2010) Use of sweet sorghum juice for lipid production by Schizochytrium limacinum SR21. Bioresource Technol 101:3623–3627

    Article  CAS  Google Scholar 

  17. Liang Y, Tang T, Siddaramu T, Choudhary R, Umagiliyage AL (2012) Lipid production from sweet sorghum bagasse through yeast fermentation. Renew Energy 40:130–136

    Article  CAS  Google Scholar 

  18. Liang Y, Tang T, Umagiliyage AL, Siddaramu T, McCarroll M, Choudhary R (2012) Utilization of sorghum bagasse hydrolysates for producing microbial lipids. Appl Energy 91:451–458

    Article  CAS  Google Scholar 

  19. Ma XJ, Li H, Wang DX, Song X (2014) Sophorolipid production from delignined corncob residue by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 and Cryptococcus curvatus ATCC 96219. Appl Microbiol Biotechnol 98:475–483

    Article  CAS  PubMed  Google Scholar 

  20. Maingault M (1999) Utilization of sophorolipids as therapeutically active substances or cosmetic products, in particular for the treatment of the skin. Google Patents, US5981497 A

  21. Mills TY, Sandoval NR, Gill RT (2009) Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli. Biotechnol Biofuels 2:1

    Article  Google Scholar 

  22. Mittelbach M, Enzelsberger H (1999) Transesterification of heated rapeseed oil for extending diesel fuel. J Am Oil Chem Soc 76:545–550

    Article  CAS  Google Scholar 

  23. Morya VK, Park JH, Kim TJ, Jeon S, Kim EK (2013) Production and characterization of low molecular weight sophorolipid under fed-batch culture. Bioresour Technol 143:282–288

    Article  CAS  PubMed  Google Scholar 

  24. Palmqvist E, Hahn-Hägerdal B (2000) Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol 74:25–33

    Article  CAS  Google Scholar 

  25. Pekin G, Vardar-Sukan F, Kosaric N (2005) Production of sophorolipids from Candida bombicola ATCC 22214 using Turkish corn oil and honey. Eng Life Sci 5:357–362

    Article  CAS  Google Scholar 

  26. Rau U, Hammen S, Heckmann R, Wray V, Lang S (2001) Sophorolipids: a source for novel compounds. Ind Crops Prod 13:85–92

    Article  CAS  Google Scholar 

  27. Samad A, Zhang J, Chen D, Liang Y (2015) Sophorolipid production from biomass hydrolysates. Appl Biochem Biotechnol 175:2246–2257

    Article  CAS  PubMed  Google Scholar 

  28. Shah V, Jurjevic M, Badia D (2007) Utilization of restaurant waste oil as a precursor for sophorolipid production. Biotechnol Prog 23:512–515

    Article  CAS  PubMed  Google Scholar 

  29. Solaiman DK, Ashby RD, Zerkowski JA, Foglia TA (2007) Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola. Biotechnol Lett 29:1341–1347

    Article  CAS  PubMed  Google Scholar 

  30. Van Bogaert INA, Saerens K, De Muynck C, Develter D, Soetaert W, Vandamme EJ (2007) Microbial production and application of sophorolipids. Appl Microbiol Biotechnol 76:23–34

    Article  PubMed  Google Scholar 

  31. Yesuf JLY-N (2012) Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology. Biomass Bioenergy 30:367–375

    Google Scholar 

  32. Zhou Q-H, Kosaric N (1995) Utilization of canola oil and lactose to produce biosurfactant withCandida bombicola. J Am Oil Chem Soc 72:67–71

    Article  CAS  Google Scholar 

  33. Zhou Q, Kosaric N (1993) Effect of lactose and olive oil on intra-and extracellular lipids of Torulopsis bombicola. Biotechnol Lett 15:477–482

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yanna Liang.

Ethics declarations

Conflict of interest

The authors declare that they have no competing and financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Samad, A., Zhang, J., Chen, D. et al. Sweet sorghum bagasse and corn stover serving as substrates for producing sophorolipids. J Ind Microbiol Biotechnol 44, 353–362 (2017). https://doi.org/10.1007/s10295-016-1891-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-016-1891-y

Keywords

Navigation