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Mussel-inspired surface modification of magnetic@graphite nanosheets composite for efficient Candida rugosa lipase immobilization

  • Biotechnology Methods
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

By the facile adhesion way, the novel composite complex by polydopamine (PDA) and magnetic graphite nanosheets (Fe3O4@GNSs) has been successfully synthesized. The resulting composite was characterized by means of scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, and Raman spectra, X-ray diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. Meanwhile, the PDA functionalized Fe3O4@GNSs (Fe3O4@GNSs-PDA) was applied for Candida rugosa lipase (CRL) immobilization covalently without any toxic coupling agent. Combining the superior physical properties and chemical stability of Fe3O4@GNSs and the well biocompatibility, functional characteristics of PDA, the Fe3O4@GNSs-PDA composite displayed several advantages, including the high enzyme capacity, enzyme activity and stability and a decrease in enzyme loss. Our work demonstrated that the mussel-inspired Fe3O4@GNSs can be extended to many other applications such as biocatalytic, genetic and industrial.

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Acknowledgments

The authors thank the financial supports from the National Natural Science Foundation of China (No. 21374045), the scientific research ability training of under-graduate students majoring in chemistry by the two patters based on the tutorial system and top students (J1103307) and the Opening Foundation of State Key Laboratory of Applied Organic Chemistry (SKLAOC-2009-35).

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The authors declare no competing financial interest.

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    Authors and Affiliations

    1. State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, Lanzhou, 730000, China

      Chen Hou, Lincheng Zhou, Hao Zhu, Xinyu Wang, Niran Hu, Fang Zeng, Liyuan Wang & Hang Yin

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    1. Chen Hou
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    Correspondence to Hao Zhu.

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    This paper is dedicated to memory of pro. Yanfeng Li, who passed away recently.

    Chen Hou and Hao Zhu contributed equally to this work.

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    Hou, C., Zhou, L., Zhu, H. et al. Mussel-inspired surface modification of magnetic@graphite nanosheets composite for efficient Candida rugosa lipase immobilization. J Ind Microbiol Biotechnol 42, 723–734 (2015). https://doi.org/10.1007/s10295-015-1602-0

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