Abstract
In this study, we prepared injectable collagen microspheres for the sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) for tissue engineering. Collagen solution was formed into microspheres under a water-in-oil emulsion condition, followed by crosslinking with water-soluble carbodiimide. Various sizes of collagen microspheres in the range of 1–30 μm diameters could be obtained by controlling the surfactant concentration and rotating speed of the emulsified mixture. Particle size proportionally decreased with increasing the rotating speed (1.8 μm per 100 rpm increase in the range of 300–1,200 rpm) and surfactant concentration (3.1 μm per 0.1% increase in the range of 0.1–0.5%). The collagen microspheres showed a slight positive charge of 8.86 and 3.15 mV in phosphate-buffered saline and culture medium, respectively. Release study showed the sustained release of rhVEGF for 4 weeks. Released rhVEGF was able to induce capillary formation of human umbilical vein endothelial cells, indicating the maintenance of rhVEGF bioactivity after release. In conclusion, the results suggest that the collagen microspheres have potential for sustained release of rhVEGF.
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Acknowledgments
This work was supported by Takeda Science Foundation and the Research for Promoting Technological Seeds from Japan Science and Technology Agency. We thank Shunji Yunoki PhD, Risa Itoh and Masanobu Munekata PhD, Hokkaido University, for helpful advices and technical assistances. We thank Sysmex for measurements of zeta potential and helpful advices.
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Nagai, N., Kumasaka, N., Kawashima, T. et al. Preparation and characterization of collagen microspheres for sustained release of VEGF. J Mater Sci: Mater Med 21, 1891–1898 (2010). https://doi.org/10.1007/s10856-010-4054-0
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DOI: https://doi.org/10.1007/s10856-010-4054-0