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Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production

  • Metabolic Engineering and Synthetic Biology
  • Published:
Journal of Industrial Microbiology & Biotechnology

An Erratum to this article was published on 03 April 2015

Abstract

(3R)-Acetoin and (2R,3R)-2,3-butanediol are important pharmaceutical intermediates. However, until now, the quantity of natural microorganisms with the ability to produce single configuration of optically pure (3R)-acetoin and (2R,3R)-2,3-butanediol is rare. In this study, a meso-2,3-butanediol dehydrogenase encoded by the slaC gene from Serratia marcescens MG1 was identified for meso-2,3-butanediol and (2S,3S)-2,3-butanediol biosynthesis. Inactivation of the slaC gene could significantly decrease meso-2,3-butanediol and (2S,3S)-2,3-butanediol and result in a large quantity of (3R)-acetoin accumulation. Furthermore, a (2R,3R)-2,3-butanediol dehydrogenase encoded by the bdhA gene from Bacillus subtilis 168 was introduced into the slaC mutant strain of Serratia marcescens MG1. Excess (2R,3R)-2,3-butanediol dehydrogenase could accelerate the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol and lead to (2R,3R)-2,3-butanediol accumulation. In fed-batch fermentation, the excess (2R,3R)-2,3-butanediol dehydrogenase expression strain could produce 89.81 g/l (2R,3R)-2,3-butanediol with a productivity of 1.91 g/l/h at 48 h. These results provided potential applications for (3R)-acetoin and (2R,3R)-2,3-butanediol production.

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Acknowledgments

This work was supported by Shanghai Leading Academic Discipline Project (project B505) and National Special Fund for State Key Laboratory of Bioreactor Engineering (no. 2060204).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Fangmin Bai, Lu Dai, Jiying Fan, Ngoctu Truong, Ben Rao & Yaling Shen

  2. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People’s Republic of China

    Liaoyuan Zhang

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  1. Fangmin Bai
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  2. Lu Dai
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  3. Jiying Fan
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  4. Ngoctu Truong
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  6. Liaoyuan Zhang
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  7. Yaling Shen
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Correspondence to Liaoyuan Zhang or Yaling Shen.

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Bai, F., Dai, L., Fan, J. et al. Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production. J Ind Microbiol Biotechnol 42, 779–786 (2015). https://doi.org/10.1007/s10295-015-1598-5

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  • DOI: https://doi.org/10.1007/s10295-015-1598-5

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