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Investigation of ptsG gene in response to xylose utilization in Corynebacterium glutamicum

  • Genetics and Molecular Biology of Industrial Organisms
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Corynebacterium glutamicum strains NC-2 were able to grow on xylose as sole carbon sources in our previous work. Nevertheless, it exhibited the major shortcoming that the xylose consumption was repressed in the presence of glucose. So far, regarding C. glutamicum, there are a number of reports on ptsG gene, the glucose-specific transporter, involved in glucose metabolism. Recently, we found ptsG had influence on xylose utilization and investigated the ptsG gene in response to xylose utilization in C. glutamicum with the aim to improve xylose consumption and simultaneously utilized glucose and xylose. The ptsG-deficient mutant could grow on xylose, while exhibiting noticeably reduced growth on xylose as sole carbon source. A mutant deficient in ptsH, a general PTS gene, exhibited a similar phenomenon. When complementing ptsG gene, the mutant ΔptsG-ptsG restored the ability to grow on xylose similarly to NC-2. These indicate that ptsG gene is not only essential for metabolism on glucose but also important in xylose utilization. A ptsG-overexpressing recombinant strain could not accelerate glucose or xylose metabolism. When strains were aerobically cultured in a sugar mixture of glucose and xylose, glucose and xylose could not be utilized simultaneously. Interestingly, the ΔptsG strain could co-utilize glucose and xylose under oxygen-deprived conditions, though the consumption rate of glucose and xylose dramatically declined. It was the first report of ptsG gene in response to xylose utilization in C. glutamicum.

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Acknowledgments

This work was supported by the 973 Program of China (Grant No. 2011CB707405).

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Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211816, China

    Chen Wang, Heng Cai, Zhihui Zhou, Kai Zhang, Yali Chen, Honggui Wan & Pingkai Ouyang

  2. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China

    Zhongjun Chen

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  1. Chen Wang
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Correspondence to Heng Cai.

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Wang, C., Cai, H., Zhou, Z. et al. Investigation of ptsG gene in response to xylose utilization in Corynebacterium glutamicum . J Ind Microbiol Biotechnol 41, 1249–1258 (2014). https://doi.org/10.1007/s10295-014-1455-y

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