Abstract
Cadaverine (1,5-pentanediamine, diaminopentane), the desired raw material of bio-polyamides, is an important industrial chemical with a wide range of applications. Biosynthesis of cadaverine in Corynebacterium glutamicum has been a competitive way in place of petroleum-based chemical synthesis method. To date, the cadaverine exporter has not been found in C. glutamicum. In order to improve cadaverine secretion, the cadaverine–lysine antiporter CadB from Escherichia coli was studied in C. glutamicum. Fusion expression of cadB and green fluorescent protein (GFP) gene confirmed that CadB could express in the cell membrane of C. glutamicum. Co-expression of cadB and ldc from Hafnia alvei in C. glutamicum showed that the cadaverine secretion rate increased by 22 % and the yield of total cadaverine and extracellular cadaverine increased by 30 and 73 %, respectively. Moreover, the recombinant strain cultured at acid and neutral pH separately hardly had any difference in cadaverine concentrations. These results suggested that CadB could be expressed in the cell membrane of C. glutamicum and that recombinant CadB could improve cadaverine secretion and the yield of cadaverine. Moreover, the pH value did not affect the function of recombinant CadB. These results may be a promising metabolic engineering strategy for improving the yield of the desired product by enhancing its export out of the cell.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 21176190), Key Technology Research and Development Program of Tianjin, China (No. 11ZCKFSY00900) and Tianjin Research Program of Application Foundation and Advanced Technology (No. 11JCYBJC09600).
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We declare that we have no financial or personal relationships with other people or organizations that could inappropriately influence our work. There is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled ‘Improving the secretion of cadaverine in Corynebacterium glutamicum by co-expressing lysine decarboxylase and cadaverine–lysine antiporter’.
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Li, M., Li, D., Huang, Y. et al. Improving the secretion of cadaverine in Corynebacterium glutamicum by cadaverine–lysine antiporter. J Ind Microbiol Biotechnol 41, 701–709 (2014). https://doi.org/10.1007/s10295-014-1409-4
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DOI: https://doi.org/10.1007/s10295-014-1409-4