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Integrated bioprocess for the stereospecific production of linalool oxides from linalool with Corynespora cassiicola DSM 62475

  • Biocatalysis
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Linalool oxides are of interest to the flavour industry because of their lavender notes. Corynespora cassiicola DSM 62475 has been identified recently as a production organism because of high stereoselectivity and promising productivities [Mirata et al. (2008) J Agric Food Chem 56(9):3287–3296]. In this work, the stereochemistry of this biotransformation was further investigated. Predominantly (2R)-configured linalool oxide enantiomers were produced from (R)-(−)-linalool. Comparative investigations with racemic linalool suggest that predominantly (2S)-configured derivatives can be expected by using (S)-(+)-configured substrate. Substrate and product inhibited growth even at low concentrations (200 mg l−1). To avoid toxic effects and supply sufficient substrates, a substrate feeding product removal (SFPR) system based on hydrophobic adsorbers was established. Applying SFPR, productivity on the shake flask scale was increased from 80 to 490 mg l−1 day−1. Process optimisation increased productivity to 920 mg l−1 day−1 in a bioreactor with an overall product concentration of 4.600 mg l−1 linalool oxides.

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Authors and Affiliations

  1. Biochemical Engineering, DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486, Frankfurt, Germany

    Sebastian Bormann, Maria M. W. Etschmann, Marco-Antonio Mirata & Jens Schrader

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  1. Sebastian Bormann
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  2. Maria M. W. Etschmann
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  3. Marco-Antonio Mirata
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  4. Jens Schrader
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Correspondence to Maria M. W. Etschmann.

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Bormann, S., Etschmann, M.M.W., Mirata, MA. et al. Integrated bioprocess for the stereospecific production of linalool oxides from linalool with Corynespora cassiicola DSM 62475. J Ind Microbiol Biotechnol 39, 1761–1769 (2012). https://doi.org/10.1007/s10295-012-1181-2

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  • DOI: https://doi.org/10.1007/s10295-012-1181-2

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