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Effects of polarization in the presence and absence of biocides on biofilms in a simulated paper machine water

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The antifouling potential of electric polarization combined and not combined with biocides was studied in nonsaline warm water with high organic content. Deinococcus geothermalis is a bacterium known for forming colored biofilms in paper machines and for its persistence against cleaning and chemical treatments. When D. geothermalis biofilms grown for 24 h in simulated paper machine water were exposed to cathodic or cathodically weighted pulsed polarization at least 60% (P < 0.05) of the biofilms were removed from stainless steel (AISI 316L). Biofilm removal by 25 ppm (effective substances 5–25 ppm) of oxidizing biocides (bromochloro-5,5-dimethylhydantoin, 2,2-dibromo-2-cyanoacetamide, peracetic acid) increased to 70% when combined with cathodically weighted pulsed polarization. Using a novel instrument that allows real-time detection of reactive oxygen species (ROS) we showed that the polarization program effective in antifouling generated ROS in a pulsed manner on the steel surface. We thus suggest that the observed added value of oxidative biocides combined with polarization depended on ROS. This suggestion was supported by the finding that a reductive biocide, methylene bisthiocyanate, counteracted the antifouling effect of polarization.

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Acknowledgments

This work was supported by the technology program PINTA of the Finnish Funding Agency for Technology and Innovation (Tekes). Additional support was obtained from Kemira Oyj, Savcor Forest Oyj, StoraEnso Oyj, UPM-Kymmene Oyj and the Center of Excellence (Photobiomics, grant no. 118637) of the Academy of Finland. We thank the Helsinki University Viikki Science Library for excellent information service, the Faculty of Agriculture and Forestry Instrument Centre for technical support, and Leena Steininger, Hannele Tukiainen, and Tuula Suortti for many kinds of help.

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Author notes

  1. Teemu Kuosmanen

    Present address: School of Puropelto, Sepänkatu 11, 20700, Turku, Finland

Authors and Affiliations

  1. Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland

    Minna Peltola, Teemu Kuosmanen, Hanna Sinkko & Mirja Salkinoja-Salonen

  2. Savcor Forest Oy, Insinöörinkatu 8, 50100, Mikkeli, Finland

    Niina Vesalainen & Martti Pulliainen

  3. UPM-Kymmene Oyj, Research Center, 53200, Lappeenranta, Finland

    Päivi Korhonen

  4. StoraEnso Oyj, Research Center, 55800, Imatra, Finland

    Kirsi Partti-Pellinen & Jari P. Räsänen

  5. Department of Forest Sciences, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    Hannu Rita

  6. Kemira Oyj, Pulp & Paper Chemicals, P.O. Box 44, 022271, Espoo, Finland

    Juha Rintala & Marko Kolari

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  1. Minna Peltola
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Correspondence to Minna Peltola.

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Peltola, M., Kuosmanen, T., Sinkko, H. et al. Effects of polarization in the presence and absence of biocides on biofilms in a simulated paper machine water. J Ind Microbiol Biotechnol 38, 1719–1727 (2011). https://doi.org/10.1007/s10295-011-0961-4

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  • DOI: https://doi.org/10.1007/s10295-011-0961-4

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