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Encapsulation of Pannonibacter phragmitetus LSSE-09 in alginate–carboxymethyl cellulose capsules for reduction of hexavalent chromium under alkaline conditions

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Journal of Industrial Microbiology & Biotechnology

Abstract

Cr(VI) was efficiently reduced to Cr(III) by Pannonibacter phragmitetus LSSE-09 encapsulated in liquid-core alginate–carboxymethyl cellulose capsules under alkaline conditions. Taking into account the physical properties of the capsules, the activity of encapsulated cells, and total Cr(III) concentration in the supernatant, optimal conditions (0.5% w/v sodium alginate; 2% w/v sodium carboxymethyl cellulose; 0.1 M CaCl2; 30-min gelation time) for LSSE-09 encapsulation were determined. At optimal conditions, a relatively high reduction rate of 4.20 mg g −1(dry weight)  min−1 was obtained. Total Cr(III) concentration in the supernatant was significantly decreased after reduction, because 63.7% of the formed soluble organo-Cr(III) compounds compared with those of free cells were captured by the relatively smaller porous structure of alginate capsules. The optimal pH value (9.0) for Cr(VI) reduction was not changed after encapsulation. In addition, encapsulated LSSE-09 showed no appreciable loss in activity after eight repeated cycles at 37°C, and 85.7% of its initial activity remained after 35-day storage at 4°C. The results suggest that encapsulated LSSE-09 in alginate–carboxymethyl cellulose capsules has potential biotechnological applications for the detoxification of Cr(VI)-contaminated wastewater.

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References

  1. Baral A, Engelken RD (2002) Chromium-based regulations and greening in metal finishing industries in the USA. Environ Sci Policy 5:121–133

    Article  CAS  Google Scholar 

  2. Barnhart J (1997) Occurrences, uses, and proprties of chromium. Regul Toxicol Pharmacol 26:S3–S7

    Article  CAS  Google Scholar 

  3. Blandino A, Macías M, Cantero D (1999) Formation of calcium alginate gel capsules: influence of sodium alginate and CaCl2 concentration on gelation kinetics. J Biosci Bioeng 88:686–689

    Article  PubMed  CAS  Google Scholar 

  4. Blandino A, Macías M, Cantero D (2000) Glucose oxidase release from calcium alginate gel capsules. Enzyme Microb Technol 27:319–324

    Article  PubMed  CAS  Google Scholar 

  5. Blandino A, Macías M, Cantero D (2001) Immobilization of glucose oxidase within calcium alginate gel capsules. Process Biochem 36:601–606

    Article  CAS  Google Scholar 

  6. Bopp LH, Ehrlich HL (1988) Chromate resistance and reduction in Pseudomonas fluorescens strain LB300. Arch Microbiol 150:426–431

    Article  CAS  Google Scholar 

  7. Bŭcko M, Vikartovská A, Lacík I, Kolláriková G, Gemeiner P, Pätoprstý V, Brygin M (2005) Immobilization of a whole-cell epoxide-hydrolyzing biocatalyst in sodium alginate-cellulose sulfate-poly (methylene-co-guanidine) capsules using a controlled encapsulation process. Enzyme Microb Technol 36:118–126

    Article  Google Scholar 

  8. Campos J, Martinez-Pacheco M, Cervantes C (1995) Hexavalent-chromium reduction by a chromate-resistant Bacillus sp. strain. Anton Van Lee 68:203–208

    Article  CAS  Google Scholar 

  9. Cassidy MB, Lee H, Trevors JT (1996) Environmental applications of immobilized microbial cells: a review. J Ind Microbiol 16:79–101

    Article  CAS  Google Scholar 

  10. Cheong SH, Park JK, Kim BS, Chang HN (1993) Microencapsulation of yeast cells in the calcium alginate membrane. Biotechnol Tech 7:879–884

    Article  CAS  Google Scholar 

  11. Costa M (2003) Potential hazards of hexavalent chromate in our drinking water. Toxicol Appl Pharmacol 188:1–5

    Article  PubMed  CAS  Google Scholar 

  12. Goosen MFA (1993) Biomedical application of immobilised cells. In: Goosen MFA (ed) Fundamentals of animal cell encapsulation and immobilization. CRC, Boca Raton

  13. Greenberg AE, Trussell RR, Clesceri LS (1985) Standard methods for the examination of water and wastewater. APHA, New York

    Google Scholar 

  14. He Z, Gao F, Sha T, Hu Y, He C (2009) Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp. strain CSCr-3 from chromium landfill. J Hazard Mater 163:869–873

    Article  PubMed  CAS  Google Scholar 

  15. Hucík M, Bučko M, Gemeiner P, Štefuca V, Vikartovská A, Mihovilovič M, Rudroff F, Iqbal N, Chorvát D, Lacík I (2010) Encapsulation of recombinant E. coli expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one. Biotechnol Lett 32:675–680

    Article  PubMed  Google Scholar 

  16. Humphries AC, Nott KP, Hall LD, Macaskie LE (2005) Reduction of Cr(VI) by immobilized cells of Desulfovibrio vulgaris NCIMB 8303 and Microbacterium sp. NCIMB 13776. Biotechnol Bioeng 90:589–596

    Article  PubMed  CAS  Google Scholar 

  17. Jiang Z, Zhang Y, Li J, Jiang W, Yang D, Wu H (2007) Encapsulation of β-glucuronidase in biomimetic alginate capsules for bioconversion of baicalin to baicalein. Ind Eng Chem Res 46:1883–1890

    Article  CAS  Google Scholar 

  18. Kathiravan MN, Karthiga Rani R, Karthick R, Muthukumar K (2010) Mass transfer studies on the reduction of Cr(VI) using calcium alginate immobilized Bacillus sp. in packed bed reactor. Bioresour Technol 101:853–858

    Article  PubMed  CAS  Google Scholar 

  19. Koyama K, Seki M (2004) Cultivation of yeast and plant cells entrapped in the low-viscous liquid-core of an alginate membrane capsule prepared using polyethylene glycol. J Biosci Bioeng 97:111–118

    PubMed  CAS  Google Scholar 

  20. Kratochvil D, Pimentel P, Volesky B (1998) Removal of trivalent and hexavalent chromium by seaweed biosorbent. Environ Sci Technol 32:2693–2698

    Article  CAS  Google Scholar 

  21. Kuo CK, Ma PX (2001) Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties. Biomaterials 22:511–521

    Article  PubMed  CAS  Google Scholar 

  22. Li Y, Xing J, Xiong X, Li W, Gao H, Liu H (2008) Improvement of biodesulfurization activity of alginate immobilized cells in biphasic systems. J Ind Microbiol Biotechnol 35:145–150

    Article  PubMed  Google Scholar 

  23. Llovera S, Bonet R, Simon-Pujol MD, Congregado F (1993) Chromate reduction by resting cells of Agrobacterium radiobacter EPS-916. Appl Environ Microbiol 59:3516–3518

    PubMed  CAS  Google Scholar 

  24. McLean J, Beveridge TJ (2001) Chromate reduction by a Pseudomonad isolated from a site contaminated with chromated copper arsenate. Appl Environ Microbiol 67:1076–1084

    Article  PubMed  CAS  Google Scholar 

  25. Middleton SS, Latmani RB, Mackey MR, Ellisman MH, Tebo BM, Criddle CS (2003) Cometabolism of Cr(VI) by Shewanella oneidensis MR-1 produces cell-associated reduced chromium and inhibits growth. Biotechnol Bioeng 83:627–637

    Article  PubMed  CAS  Google Scholar 

  26. Moreno-Garrido I (2008) Microalgae immobilization: current techniques and uses. Bioresour Technol 99:3949–3964

    Article  PubMed  CAS  Google Scholar 

  27. Nigam SC, Tsao IF, Sakoda A, Wang HY (1988) Techniques for preparing hydrogel membrane capsules. Biotechnol Tech 2:271–276

    Article  CAS  Google Scholar 

  28. Okutucu B, Çelem EB, Önal S (2010) Immobilization of α-galactosidase on galactose-containing polymeric beads. Enzyme Microb Technol 46:200–205

    Article  CAS  Google Scholar 

  29. Pattanapipitpaisal P, Brown NL, Macaskie LE (2001) Chromate reduction by Microbacterium liquefaciens immobilised in polyvinyl alcohol. Biotechnol Lett 23:61–65

    Article  CAS  Google Scholar 

  30. Patterson JW (1975) Wastewater treatment technology. Ann Arbor Science, New York

    Google Scholar 

  31. Peng Q, Liu Y, Zeng G, Xu W, Yang C, Zhang J (2010) Biosorption of copper (II) by immobilizing Saccharomyces cerevisiae on the surface of chitosan-coated magnetic nanoparticles from aqueous solution. J Hazard Mater 177:676–682

    Article  PubMed  CAS  Google Scholar 

  32. Puzon GJ, Roberts AG, Kramer DM, Xun L (2005) Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics. Environ Sci Technol 39:2811–2817

    Article  PubMed  CAS  Google Scholar 

  33. Rai D, Sass BM, Moore DA (1987) Chromium(III) hydrolysis constants and solubility of chromium(III) hydroxide. Inorg Chem 26:345–349

    Article  CAS  Google Scholar 

  34. Shen H, Wang YT (1993) Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456. Appl Environ Microbiol 59:3771–3777

    PubMed  CAS  Google Scholar 

  35. SmidsrØd O, Skjåk-Bræk G (1990) Alginate as immobilization matrix for cells. Trends Biotechnol 8:71–78

    Article  PubMed  Google Scholar 

  36. Srivastava S, Thakur I (2007) Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent. Biodegradation 18:637–646

    Article  PubMed  CAS  Google Scholar 

  37. Sultan S, Hasnain S (2007) Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals. Bioresour Technol 98:340–344

    Article  PubMed  CAS  Google Scholar 

  38. Tanriseven A, Dogan S (2001) Immobilization of invertase within calcium alginate gel capsules. Process Biochem 36:1081–1083

    Article  CAS  Google Scholar 

  39. Wang X, Gai Z, Yu B, Feng J, Xu C, Yuan Y, Lin Z, Xu P (2007) Degradation of carbazole by microbial cells immobilized in magnetic gellan gum gel beads. Appl Environ Microbiol 73:6421–6428

    Article  PubMed  CAS  Google Scholar 

  40. Xu L, Luo M, Li W, Wei X, Xie K, Liu L, Jiang C, Liu H (2011) Reduction of hexavalent chromium by Pannonibacter phragmitetus LSSE-09 stimulated with external electron donors under alkaline conditions. J Hazard Mater 185:1169–1176

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Basic Research Program of China (No. 2007CB613507).

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Authors and Affiliations

  1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Bei Er Tiao, Zhong Guan Cun, Haidian District, Beijing, 100190, China

    Lin Xu, Liangrong Yang, Xuetuan Wei & Huizhou Liu

  2. National Key Laboratory of Biochemical Engineering, Chinese Academy of Sciences, Beijing, 100190, China

    Lin Xu, Liangrong Yang, Xuetuan Wei & Huizhou Liu

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Lin Xu, Mingfang Luo, Xuetuan Wei & Xing Lin

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  1. Lin Xu
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  2. Mingfang Luo
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  3. Liangrong Yang
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  6. Huizhou Liu
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Correspondence to Huizhou Liu.

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Xu, L., Luo, M., Yang, L. et al. Encapsulation of Pannonibacter phragmitetus LSSE-09 in alginate–carboxymethyl cellulose capsules for reduction of hexavalent chromium under alkaline conditions. J Ind Microbiol Biotechnol 38, 1709–1718 (2011). https://doi.org/10.1007/s10295-011-0960-5

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  • DOI: https://doi.org/10.1007/s10295-011-0960-5

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