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Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Pretreatment is a necessary step in the biomass-to-ethanol conversion process. The side stream of the pretreatment step is the liquid fraction, also referred to as the hydrolyzate, which arises after the separation of the pretreated solid and is composed of valuable carbohydrates along with compounds that are potentially toxic to microbes (mainly furfural, acetic acid, and formic acid). The aim of our study was to utilize the liquid fraction from steam-exploded wheat straw as a carbon source for cellulase production by Trichoderma reesei RUT C30. Results showed that without detoxification, the fungus failed to utilize any dilution of the hydrolyzate; however, after a two-step detoxification process, it was able to grow on a fourfold dilution of the treated liquid fraction. Supplementation of the fourfold-diluted, treated liquid fraction with washed pretreated wheat straw or ground wheat grain led to enhanced cellulase (filter paper) activity. Produced enzymes were tested in hydrolysis of washed pretreated wheat straw. Supplementation with ground wheat grain provided a more efficient enzyme mixture for the hydrolysis by means of the near-doubled β-glucosidase activity obtained.

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References

  1. Almeida JRM, Modig T, Petersson A, Hähn-Hägerdal B, Lidén G, Gorwa-Grauslund MF (2007) Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. J Chem Technol Biotechnol 82:340–349. doi:10.1002/jctb.1676

    Article  CAS  Google Scholar 

  2. Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101:4851–4861. doi:10.1016/j.biortech.2009.11.093

    Article  PubMed  CAS  Google Scholar 

  3. Ballesteros I, Negro MJ, Oliva JM, Cabanas A, Manzanares P, Ballesteros M (2006) Ethanol production from steam-explosion pretreated wheat straw. Appl Biochem Biotechnol 129–132:496–508. doi:10.1385/ABAB:130:1:496

    Article  PubMed  Google Scholar 

  4. Benitez T, Limón C, Delgado-Jarana J, Rey M (1998) Glucanolytic and other enzymes and their genes. In: Harman GE, Kubicek CP (eds) Trichoderma and gliocladium, vol 2. Taylor & Francis, London, UK, pp 89–113

    Google Scholar 

  5. Berghem LE, Pettersson LG (1974) The mechanism of enzymatic cellulose degradation. Isolation and some properties of a β-glucosidase from Trichoderma viride. Eur J Biochem 46:295–305. doi:10.1111/j.1432-1033.1974.tb03621.x

    Article  PubMed  CAS  Google Scholar 

  6. Berlin A, Gilkes N, Kilburn D, Bura R, Markov A, Skomarovsky A, Okunev O, Gusakov A, Maximenko V, Gregg D, Sinitsyn A, Saddler J (2005) Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates—evidence for the role of accessory enzymes. Enzyme Microb Technol 37:175–184. doi:10.1016/j.enzmictec.2005.01.039

    Article  CAS  Google Scholar 

  7. Bigelow M, Wyman C (2002) Cellulase production on bagasse pretreated with hot water. Appl Biochem Biotechnol 98–100:921–934. doi:10.1385/ABAB:98-100:1-9:921

    Article  PubMed  Google Scholar 

  8. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3

    Article  PubMed  CAS  Google Scholar 

  9. Breuil C, Chan M, Gilbert M, Saddler JN (1992) Influence of β-glucosidase on the filter paper activity and hydrolysis of lignocellulosic substrates. Bioresour Technol 39:139–142. doi:10.1016/0960-8524(92)90132-H

    Article  CAS  Google Scholar 

  10. Chen S, Wayman M (1992) Novel inducers derived from starch for cellulase production by Trichoderma reesei. Process Biochem 27:327–334. doi:10.1016/0032-9592(92)87010-E

    Article  CAS  Google Scholar 

  11. Fujimoto H, Nishida H, Ajisaka K (1988) Enzymatic syntheses of glucobioses by a condensation reaction with α-glucosidase, β-glucosidases and glucoamylase. Agric Biol Chem 52:1345–1351

    CAS  Google Scholar 

  12. Hayward T, Hamilton J, Templeton D, Jennings E, Ruth M, Tholudur A, McMillan J, Tucker M, Mohagheghi A (1999) Enzyme production, growth, and adaptation of T. reesei strains QM9414, L-27, RL-P37, and RUT C-30 to conditioned yellow poplar sawdust hydrolysate. Appl Biochem Biotechnol 77:293–309. doi:10.1385/ABAB:77:1-3:293

    Article  Google Scholar 

  13. Hägglund E (1951) The wood components and their chemical properties. In: Hägglund E (ed) Chemistry of wood. Academic, New York, USA, pp 37–389

    Google Scholar 

  14. Horváth IS, Sjöde A, Alriksson B, Jönsson LJ, Nilvebrant N (2005) Critical conditions for improved fermentability during overliming of acid hydrolysates from spruce. Appl Biochem Biotechnol 121–124:1031–1044. doi:10.1007/978-1-59259-991-2_87

    Article  PubMed  Google Scholar 

  15. Ilmén M, Thrane C, Penttilä M (1996) The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Mol Gen Genet 251:451–460. doi:10.1007/BF02172374

    PubMed  Google Scholar 

  16. Jorgensen H, Kristensen JB, Felby C (2007) Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities. Biofuels Bioprod Biorefin 1:119–134. doi:10.1002/bbb.4

    Article  Google Scholar 

  17. Juhász T, Szengyel Z, Szijártó N, Réczey K (2004) Effect of pH on cellulase production of Trichoderma reesei RUT C30. Appl Biochem Biotechnol 113:201–211. doi:10.1385/ABAB:113:1-3:201

    Article  PubMed  Google Scholar 

  18. Kaparaju P, Serrano M, Thomsen AB, Kongjan P, Angelidaki I (2009) Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour Technol 100:2562–2568. doi:10.1016/j.biortech.2008.11.011

    Article  PubMed  CAS  Google Scholar 

  19. Kaparaju P, Serrano M, Angelidaki I (2009) Effect of reactor configuration on biogas production from wheat straw hydrolysate. Bioresour Technol 100:6317–6323. doi:10.1016/j.biortech.2009.06.101

    Article  PubMed  CAS  Google Scholar 

  20. Kovács K, Szakacs G, Zacchi G (2009) Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride. Bioresour Technol 100:1350–1357. doi:10.1016/j.biortech.2008.08.006

    Article  PubMed  Google Scholar 

  21. Kumar S, Singh SP, Mishra IM, Adhikari DK (2009) Recent advances in production of bioethanol from lignocellulosic biomass. Chem Eng Technol 32:517–526. doi:10.1002/ceat.200800442

    Article  CAS  Google Scholar 

  22. Larsson S, Reimann A, Nilvebrant N, Jönsson L (1999) Comparison of different methods for the detoxification of lignocellulose hydrolyzates of spruce. Appl Biochem Biotechnol 77:91–103. doi:10.1385/ABAB:77:1-3:91

    Article  Google Scholar 

  23. Lo C, Zhang Q, Lee P, Ju L (2005) Cellulase production by Trichoderma reesei using sawdust hydrolysate. Appl Biochem Biotechnol 121–124:561–573. doi:10.1385/ABAB:122:1-3:0561

    Article  PubMed  Google Scholar 

  24. Marchessault RH, Malhotra SL, Jones AY, Perovic A (1983) The wood explosion process: characterization and uses of lignin/cellulose products. In: Wood and agriculture residues. Academic, New York, pp 401–413

  25. Marquina D, Flores M (1997) Use of potato starch extraction wastes to grow cellulolytic fungi. Adv Food Sci 19:75–80

    Google Scholar 

  26. Messner R, Kubicek CP (1990) Evidence for a single, specific β-glucosidase in cell walls from Trichoderma reesei QM9414. Enzyme Microb Technol 12:685–690. doi:10.1016/0141-0229(90)90008-E

    Article  CAS  Google Scholar 

  27. Mohagheghi A, Grohmann K, Wyman C (1988) Production of cellulase on mixtures of xylose and cellulose. Appl Biochem Biotechnol 17:263–277. doi:10.1007/BF02779162

    Article  CAS  Google Scholar 

  28. Nogawa M, Goto M, Okada H, Morikawa Y (2001) l-Sorbose induces cellulase gene transcription in the cellulolytic fungus Trichoderma reesei. Curr Genet 38:329–334. doi:10.1007/s002940000165

    Article  PubMed  CAS  Google Scholar 

  29. Olsson L, Hahn-Hägerdal B (1996) Fermentation of lignocellulosic hydrolysates for ethanol production. Enzyme Microb Technol 18:312–331. doi:10.1016/0141-0229(95)00157-3

    Article  CAS  Google Scholar 

  30. Palmqvist E, Hahn-Hägerdal B, Szengyel Z, Zacchi G, Réczey K (1997) Simultaneous detoxification and enzyme production of hemicellulose hydrolysates obtained after steam pretreatment. Enzyme Microb Technol 20:286–293. doi:10.1016/S0141-0229(96)00130-5

    Article  CAS  Google Scholar 

  31. Palmqvist E, Hahn-Hägerdal B (2000) Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification. Bioresour Technol 74:17–24. doi:10.1016/S0960-8524(99)00160-1

    Article  CAS  Google Scholar 

  32. Parajó JC, Domínguez H, Domínguez JM (1998) Biotechnological production of xylitol part. 3: Operation in culture media made from lignocellulose hydrolysates. Bioresour Technol 66:25–40. doi:10.1016/S0960-8524(98)00037-6

    Article  Google Scholar 

  33. Réczey K, Brumbauer A, Bollók M, Szengyel Z, Zacchi G (1998) Use of hemicellulose hydrolysate for β-glucosidase fermentation. Appl Biochem Biotechnol 70–72:225–235. doi:10.1007/BF02920139

    Article  Google Scholar 

  34. Seidl V, Gamauf C, Druzhinina IS, Seiboth B, Hartl L, Kubicek CP (2008) The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome. BMC Genomics 9:327. doi:10.1186/1471-2164-9-327

    Article  PubMed  Google Scholar 

  35. Sternberg D, Mandels GR (1979) Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol 139:761–769

    PubMed  CAS  Google Scholar 

  36. Szengyel Z, Zacchi G, Réczey K (1997) Cellulase production based on hemicellulose hydrolysate from steam-pretreated willow. Appl Biochem Biotechnol 63–65:351–362. doi:10.1007/BF02920437

    Article  PubMed  Google Scholar 

  37. Szengyel Z, Zacchi G (2000) Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30. Appl Biochem Biotechnol 89:31–42. doi:10.1385/ABAB:89:1:31

    Article  PubMed  CAS  Google Scholar 

  38. Taj-Aldeen SJ (1993) Effect of starch on the induction of β-glucosidase in Trichoderma reesei. Mycol Res 97:318–320. doi:10.1016/S0953-7562(09)81128-3

    Article  CAS  Google Scholar 

  39. Talebnia F, Karakashev D, Angelidaki I (2010) Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentation. Bioresour Technol 101:4744–4753. doi:10.1016/j.biortech.2009.11.080

    Article  PubMed  CAS  Google Scholar 

  40. van Maris AJA, Abbott DA, Bellissimi E, van den Brink J, Kuyper M, Luttik MAH, Wisselink HW, Scheffers WA, van Dijken JP, Pronk JT (2006) Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status. Antonie van Leeuwenhoek 90:391–418. doi:10.1007/s10482-006-9085-7

    Article  PubMed  CAS  Google Scholar 

  41. Wang CH, Hseu TH, Huang CM (1988) Induction of cellulase by cello-oligosaccharides in Trichoderma koningii G-39. J Biotechnol 9:47–59. doi:10.1016/0168-1656(88)90014-4

    Article  Google Scholar 

  42. Warzywoda M, Larbre E, Pourquié J (1992) Production and characterization of cellulolytic enzymes from Trichoderma reesei grown on various carbon sources. Bioresour Technol 39:125–130. doi:10.1016/0960-8524(92)90130-P

    Article  CAS  Google Scholar 

  43. Wayman M, Chen S (1992) Cellulase production by Trichoderma reesei using whole wheat flour as a carbon source. Enzyme Microb Technol 14:825–831. doi:10.1016/0141-0229(92)90099-A

    Article  CAS  Google Scholar 

  44. Xiong H, Turunen O, Pastinen O, Leisola M, von Weymarn N (2004) Improved xylanase production by Trichoderma reesei grown on l-arabinose and lactose or d-glucose mixtures. Appl Microbiol Biotechnol 64:353–358. doi:10.1007/s00253-003-1548-4

    Article  PubMed  CAS  Google Scholar 

  45. Xiong H, von Weymarn N, Turunen O, Leisola M, Pastinen O (2005) Xylanase production by Trichoderma reesei RUT C-30 grown on l-arabinose-rich plant hydrolysates. Bioresour Technol 96:753–759. doi:10.1016/j.biortech.2004.08.007

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

Hungarian National Research Fund (OTKA K72710) is gratefully acknowledged for the financial support.

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Authors and Affiliations

  1. Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Szent Gellért tér 4, 1111, Budapest, Hungary

    Miklós Gyalai-Korpos, Réka Mangel, Dóra Dienes & Kati Réczey

  2. CIEMAT, Renewable Energies Division, Biofuels Unit, Av. Complutense 22, 28040, Madrid, Spain

    Pablo Alvira & Mercedes Ballesteros

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  1. Miklós Gyalai-Korpos
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  2. Réka Mangel
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Correspondence to Miklós Gyalai-Korpos.

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Gyalai-Korpos, M., Mangel, R., Alvira, P. et al. Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes. J Ind Microbiol Biotechnol 38, 791–802 (2011). https://doi.org/10.1007/s10295-010-0811-9

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