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Variations of two pools of glycogen and carbohydrate in Saccharomyces cerevisiae grown with various ethanol concentrations

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Glycogen, a major reservoir of energy in Saccharomyces cerevisiae, is found to be present as soluble and membrane-bound insoluble pools. Yeast cells can store excess glycogen when grown in media with higher concentration of sugar or when subjected to nutritional stress conditions. Saccharomyces cerevisiae NCIM-3300 was grown in media having ethanol concentrations up to 12% (v/v). The effects of externally added ethanol on glycogen and other carbohydrate content of yeast were studied by using alkali digestion process. Fermentative activities of cells grown in the presence of various ethanol concentrations (2–8% v/v) exhibited increase in values of glycogen and other carbohydrate, whereas cells grown with higher concentrations of ethanol (10–12% v/v) exhibited depletion in glycogen and carbohydrate content along with decrease in cell weight. Such inhibitory effect of ethanol was also exhibited in terms of reduction in total cell count of yeast grown in media with 2–16% (v/v) ethanol and 8% (w/v) sugar. These data suggest that, as the plasma membrane is a prime target for ethanol action, membrane-bound insoluble glycogen might play a protective role in combating ethanol stress. Elevated level of cell-surface α-glucans in yeast grown with ethanol, as measured by using amyloglucosidase treatment, confirms the correlation between ethanol and glycogen.

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Acknowledgments

We are grateful to Shivaji University, Kolhapur for a grant from the Departmental Research Fellowship Scheme and for providing the research facilities to carry out this work.

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Authors and Affiliations

  1. Department of Biochemistry, Shivaji University, Kolhapur, India

    M. S. Dake, J. P. Jadhv & N. B. Patil

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  1. M. S. Dake
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  2. J. P. Jadhv
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  3. N. B. Patil
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Correspondence to M. S. Dake.

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Dake, M.S., Jadhv, J.P. & Patil, N.B. Variations of two pools of glycogen and carbohydrate in Saccharomyces cerevisiae grown with various ethanol concentrations. J Ind Microbiol Biotechnol 37, 701–706 (2010). https://doi.org/10.1007/s10295-010-0713-x

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  • DOI: https://doi.org/10.1007/s10295-010-0713-x

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