Abstract
Low-energy ion beam irradiation (10–200 keV) has been proved to have a wide range of biological effects in recent years. When Rhizopus oryzae PW352 was irradiated with a 15-keV low-energy ion beam an l(+)-lactic acid high-yield mutant, RQ4015, was obtained. When 150 g/l glucose was used as the sole carbon source, l(+)-lactic acid of RQ4015 reached 121 g/l after 36 h shake-flask cultivation. However, the highest lactic acid concentration 74 g/l was obtained when 100 g/l xylose was present in the medium as the sole carbon source. When mixed xylose (25 g/l) and glucose (75 g/l) were present in a bubble column, l(+)-lactic acid production of RQ4015 reached 83 g. A high mutation rate and a wide mutation spectrum of low-energy ion implantation were observed in the experiment, suggesting that ion implantation can be a highly efficient mutagenic means for microorganism breeding in many commercial applications.
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Acknowledgments
This work is sponsored by The National High Technology Research and Development Program (“863” Program) of China no. 2009AA02Z305. The authors thank Mr Yuan Hang in the Key Laboratory of Ion Beam Bioengineering of the Chinese Academy of Science for his help in performing the ion implantation.
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Wang, P., Li, J., Wang, L. et al. l(+)-Lactic acid production by co-fermentation of glucose and xylose with Rhizopus oryzae obtained by low-energy ion beam irradiation. J Ind Microbiol Biotechnol 36, 1363–1368 (2009). https://doi.org/10.1007/s10295-009-0621-0
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DOI: https://doi.org/10.1007/s10295-009-0621-0