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The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A variety of raw materials have been used in fermentation process. This study shows the use of rice straw hemicellulosic hydrolysate, as the only source of nutrient, to produce high added-value products. In the present work, the activity of the enzymes xylose reductase (XR); xylitol dehydrogenase (XD); and glucose-6-phosphate dehydrogenase (G6PD) during cultivation of Candida guilliermondii on rice straw hemicellulosic hydrolysate was measured and correlated with xylitol production under different pH values (around 4.5 and 7.5) and initial xylose concentration (around 30 and 70 g l−1). Independent of the pH value and xylose concentration evaluated, the title of XD remained constant. On the other hand, the volumetric activity of G6PD increased whereas the level of XR decreased when the initial xylose concentration was increased from 30 to 70 g l−1. The highest values of xylitol productivity (Q P ≈ 0.40 g l−1) and yield factor (Y P/S ≈ 0.60 g g−1) were reached at highest G6PD/XR ratio and lowest XR/XD ratio. These results suggest that NADPH concentrations influence the formation of xylitol more than the activity ratios of the enzymes XR and XD. Thus, an optimal rate between G6PD and XR must be reached in order to optimize the xylitol production.

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Acknowledgments

The authors acknowledge financial assistance from Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES (Brazil).

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Authors and Affiliations

  1. Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Estrada Municipal do Campinho s/n, P.O. Box 116, Lorena, SP, 12602-810, Brazil

    Daniela B. Gurpilhares, Francislene A. Hasmann & Inês C. Roberto

  2. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16.S, São Paulo, SP, 05508-000, Brazil

    Adalberto Pessoa Jr

Authors
  1. Daniela B. Gurpilhares
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  2. Francislene A. Hasmann
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  3. Adalberto Pessoa Jr
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  4. Inês C. Roberto
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Correspondence to Inês C. Roberto.

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Gurpilhares, D.B., Hasmann, F.A., Pessoa, A. et al. The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate. J Ind Microbiol Biotechnol 36, 87–93 (2009). https://doi.org/10.1007/s10295-008-0475-x

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  • DOI: https://doi.org/10.1007/s10295-008-0475-x

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