Abstract
The effect of phosphate (P i ) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08–0.45 h−1. The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h−1 has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P i transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08–0.1 h−1, corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.
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Acknowledgments
We gratefully acknowledge Prof. Bengt L. Persson (Kalmar and Stockholm Universities, Sweden) for acting as a co-advisor for the first author in her PhD program and for providing laboratory facilities and yeast strain. We are deeply grateful for the valuable assistance of Dr. Jens O. Lagerstedt (University of California-Davis, USA) in the western blot analyses. The authors are grateful for financial support from The Ministry of Science, Research and Technology of Iran, the KK Foundation, Human Frontier Science Organization, the Swedish Natural Science Research Council, and Växjö University, Sweden. Technical assistance of Helena Rupar is acknowledged.
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Shokrollahzadeh, S., Bonakdarpour, B., Vahabzadeh, F. et al. Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions. J Ind Microbiol Biotechnol 34, 17–25 (2007). https://doi.org/10.1007/s10295-006-0157-5
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DOI: https://doi.org/10.1007/s10295-006-0157-5