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Development of a process for the production of the anticancer lead compound pleurotin by fermentation of Hohenbuehelia atrocaerulea

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Journal of Industrial Microbiology and Biotechnology

Abstract

Pleurotin is a naphthoquinone antibiotic originally isolated from Pleurotus griseus. Two pleurotin producing strains of Hohenbuehelia atrocaerulea have been identified, which, on solid substrate fermentation for 2 months yield 1–2 mg/l of the antibiotic. Described here is the lengthy developmental process which resulted in a production protocol being developed which reliably yields pleurotin from liquid fermentation at >300 mg/l. Critical to obtaining this increase in titer was inclusion in the media of an aqueous extract of alder wood.

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Notes

  1. In the manufacturing of both Silvacel and Mat Wood #116 there is a sterilization step. The woods used to produce the data described in Fig. 3, and additional fermentations not described in Fig. 3, came from a number of sources, including branches of trees, green sawn wood, and kiln-dried lumber

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Acknowledgements

Ms. Sharon Wiles, SAIC Frederick, Inc., for excellent performance over many years in carrying through with these many fungal fermentations, and Mr. Andrew Burnett, SAIC Frederick, Inc., for outstanding isolation and analysis development early in these studies. A portion of this work was presented at the Society for Industrial Microbiology meeting, Anaheim, CA, 27 July 2004. Thanks to MAT, Inc. Floodwood, MN (http://www.soilguard.com) for providing Mat Wood #133 and #116. Thanks to McCutcheon Apple Company, Frederick, MD for providing SILVACEL, which was formerly a product of Weyerhaeuser, Inc. The content of this publication does not necessarily reflect the views or the policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1-CO-12400.

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Authors and Affiliations

  1. Natural Products Support Group, SAIC-Frederick, Inc, Bldg. 431, Box B, Frederick, MD, 21702, USA

    S. M. Shipley, A. L. Barr, S. J. Graf, R. P. Collins & T. G. McCloud

  2. Natural Products Branch, Developmental Therapeutics Program, National Cancer Institute at Frederick, Frederick, MD, 21702, USA

    D. J. Newman

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  1. S. M. Shipley
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  2. A. L. Barr
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  3. S. J. Graf
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  4. R. P. Collins
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  5. T. G. McCloud
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  6. D. J. Newman
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Correspondence to T. G. McCloud.

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Shipley, S., Barr, A., Graf, S. et al. Development of a process for the production of the anticancer lead compound pleurotin by fermentation of Hohenbuehelia atrocaerulea . J IND MICROBIOL BIOTECHNOL 33, 463–468 (2006). https://doi.org/10.1007/s10295-006-0089-0

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  • DOI: https://doi.org/10.1007/s10295-006-0089-0

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