Abstract.
We examined the performance of Streptomyces lividans strain W25 containing a hybrid expandase (deacetoxycephalosporin C synthase; DAOCS) gene, obtained by in vivo recombination between the expandase genes of S. clavuligerus and Nocardia lactamdurans for resting-cell bioconversion of penicillin G to deacetoxycephalosporin G. Strain W25 carried out a much more effective level of bioconversion than the previously used strain, S. clavuligerus NP1. The two strains also differed in the concentrations of FeSO4 and α-ketoglutarate giving maximal activity. Whereas NP1 preferred 1.8 mM FeSO4 and 1.3 mM α-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO4 and 1.9 mM α-ketoglutarate.
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Acknowledgements
This work was supported by Antibioticos, Milan, Italy. We appreciate the interest and encouragement of Ermanno Bernasconi. Thanks are given to Aiqi Fang for assistance and advice. We also acknowledge the gifts used for general support of this laboratory from the following companies: ADM, Fujisawa Pharmaceutical Co. Ltd., Kao Corporation, Meiji Seika Kaisha Ltd., Pfizer Inc., Schering-Plough Research Institute, Wyeth Research and Yamasa Corporation.
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Gao, Q., Piret, J.M., Adrio, J.L. et al. Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G. J IND MICROBIOL BIOTECHNOL 30, 190–194 (2003). https://doi.org/10.1007/s10295-003-0034-4
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DOI: https://doi.org/10.1007/s10295-003-0034-4